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Effect of different organic culture media to callus formation of anthurium / by Caroline Marero Calvelo.

By:

Calvelo, Caroline Marero [author]

Contributor(s):

Ersando, Eleanor G [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Don Severino Agricultural College, 1995.Description: 93 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

635.9 C13 1995

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

Science High School, College of Education (CED)

Abstract: CALVELO , CAROLINE M. , Laboratory School, School of Education of the Don Severino Agricultural College , Indang, Cavite, March, 1995. "EFFECT OF DIFFERENT CULTURE MEDIA TO CALLUS FORMATION OF ANTHIJRIIJM TISSUE IN TISSUE CULTURE OF ANTHIJRIUM" . Research Adviser: Mrs. Eleanor Ersando This study was conducted at the Ecosystems Research and Development Bureau, UP LB College, Laguna from May 5, 1994, to October 2, 1994. In this study , the primary objectives were to develop technique of mass propagation of anthuriums and to investigation the effects of the different organic culture media to callus formation of anthurium explants. It also aimed to determine which of these culture media is best suited for the culture of anthurium explants. Moreover, it also sought to determine ways of reducing percentage contamination among explants. The study was arranged in a Completely Randomized Design (CRD) with 6 treatments and with IØ replications. The treatments were: T — control treatment (MS medium only), T 1 (Coconut water sucrose agar), T 2 (Rice bran sucrose agar), T 3 (Sweet Potato sucrose Agar), T 4 (Banana sucrose agar), and T 5 (Tomato homogenate sucrose agar) . Results of the trial— experiment proved that the most effective length of time for surface disinfection was 2Ø-minute surface disinfection of explants on a 5% sodium hypochlorite. Results of the actual experiment which lasted for 45 days revealed that highly significant results were obtained in all the parameters used in this study. It appears that most effective culture media that can support callus growth are coconut water sucrose agar and tomato homogenate were observed on organic culture as compared to MS medium only.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	635.9 C13 1995 (Browse shelf(Opens below))		Link to resource	Room use only	R-208		00000704

Research Study (Agricultural Science Curriculum) Don Severino Agricultural College.

Includes bibliographical references.

Science High School, College of Education (CED)

CALVELO , CAROLINE M. , Laboratory School, School of Education of the Don Severino Agricultural College , Indang, Cavite, March, 1995. "EFFECT OF DIFFERENT CULTURE MEDIA TO CALLUS FORMATION OF ANTHIJRIIJM TISSUE IN TISSUE CULTURE OF ANTHIJRIUM" .
Research Adviser: Mrs. Eleanor Ersando
This study was conducted at the Ecosystems Research and Development Bureau, UP LB College, Laguna from May 5, 1994, to October 2, 1994. In this study , the primary objectives were to develop technique of mass propagation of anthuriums and to investigation the effects of the different organic culture media to callus formation of anthurium explants. It also aimed to determine which of these culture media is best suited for the culture of anthurium explants. Moreover, it also sought to determine ways of reducing percentage contamination among explants.
The study was arranged in a Completely Randomized Design (CRD) with 6 treatments and with IØ replications. The treatments were: T — control treatment (MS medium only), T 1 (Coconut water sucrose agar), T 2 (Rice bran sucrose agar), T 3 (Sweet Potato sucrose Agar), T 4 (Banana sucrose agar), and T 5 (Tomato homogenate sucrose agar) .
Results of the trial— experiment proved that the most effective length of time for surface disinfection was 2Ø-minute surface disinfection of explants on a 5% sodium hypochlorite.
Results of the actual experiment which lasted for 45 days revealed that highly significant results were obtained in all the parameters used in this study. It appears that most effective culture media that can support callus growth are coconut water sucrose agar and tomato homogenate were observed on organic culture as compared to MS medium only.

Submitted to the University Library R-208