Efficacy of Bermuda grass (Cynodon dactylon) in healing second degree burns of male albino rats (Rattus albus) / by Glaiza C. Borromeo, Keith F. Cariño and Life Arielle Escapalao.

Research Study (Bachelor of Science in Medical Technology) Cavite State University.

Includes bibliographical references.

College of Nursing (CON), Department of Medical Technology

BORROMEO, GLAIZA C., CARINO, KEITH F., AND ESCAPALAO, LIFE ARIELLE E. Efficacy of Bermuda grass (Cynodon dactylon) in healing second degree burn of male albino rat (Rattus aibus).Undergraduate Research Paper. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. November 2017. Adviser: Mr. Emmanuel D. Mago, DvM, MS and Tech-Critic: Charmaine E. Rosales, RMT.

This study was conducted from August to October 2017 at the Department of Veterinary Medicine, Cavite State University, Indang, Cavite to determine the efficacy of Bermuda grass (Cynodon dactylon) in healing second degree burn of male albino rat (Rattus albus). Specifically, the study aimed to 1) compare the healing potential of the different concentrations of Bermuda grass ethanol extracts based on percent wound closure, reducing (erythema, exudates and swelling); 2) determine the concentration of Bermuda grass ethanol extract with the best burn wound healing ability; 3) compare the Bermuda grass ethanol extract to positive control (Silver sulfadiazine) in terms of healing second degree burn. Experimental research design was used in this study. Plant extracts of Cynodon dactylon leaves were collected at General Mariano Alvarez, Cavite and prepared by washing for three times and air-drying for 15 days until crispy. Leaves were then hand-pounded, pulverized using electric blender and were sieved to obtained fine powder. Two hundred grams of each pulverized leaves were soaked in 1 liter ethanol for three days and filtered using cheesecloth and filter paper. The filtrates were then subjected to air-drying to remove traces of solvents. Different concentration were prepared by dissolving the dried extract using Carbopol 940, methyl paraben, glycerin, triethanolamine and distilled water then shake until it is gel in appearance. The treatments and corresponding concentrations are Treatment 3 (7g of Bermuda grass powdered extract), Treatment 2 (5g of Bermuda grass powdered extract), and Treatment 1 (2g of Bermuda grass powdered extract) ethanol extracts. Thirty, one year old, 250-300g, male Rattus albus were used in the experiment. Five treatments including the positive (Silver sulfadiazine) and negative control with two replicates had undergone to compare the efficacy of the different concentrations of ethanol extracts within one week. The rats were shaved in the lumbar lateral part and disinfect with alcohol before anesthetizing them. 1.5cm metal rod was heated through direct fire and applied to the rat for 10 seconds and obtained a second degree burn. Each rat were treated everyday with their corresponding treatments and observed if there is a progress in terms of erythema, swelling, exudates and wound closure. There is a healing potential on different concentrations of Bermuda grass ethanol extracts in second degree bum wound. Positive control is the most effective in wound closure and reducing erythema. All the treatments are equally effective in reducing exudates. Treatment 3 (7g) and Positive control are the most effective in terms of reducing the extent of swelling. Treatment 3 (7g) ethanol extract of Bermuda grass is the best concentration in healing second degree burn wound. Positive control (Silver sulfadiazine) was still the best treatment for the bum and it is equally effective as the 5 and 7g of Bermuda grass ethanol extract in healing second degree burn.

Submitted to the University Library July 16, 2018 RS-833