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Nutritional requirements of white lauan (Shore contorta Vid) for callus initiation / by Portia Gamboa Lapitan

By:

Lapitan, Portia Gamboa [author]

Material type: Text

TextLanguage: English Publication details: Los Baños, Laguna : 1983. Cavite State University- Main Campus,Description: 76 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

634.9 L31 1983

Online resources:

Click here to view thesis abstract and table of contents

Abstract: LAPITAN, PORTIA G. University of the Philippines at Los Bafios, May, 1983. Nutritional Requirements of White Lauan (Shorea _contorta Vid.) for Callus InitiaTion. Major Professor: Dr. M. U. Garcia Eleven culture media were tested to induce callus initiation on sprout and shoot explants of white lauan (Shorea contorta Vid.). Only 3 media were found to induce callus initiation and the best was medium IX, the basal medium of Murashige and Skoog supplemented with myoinositol (100 mg/1), nicotinic acid (0.5 mg/1), thiamine-HC1 (0.1 mg/1), pyridoxine-HC1 (0.1 mg/l), choline chloride (10 mg/1), calcium pantothenate (0.1 mg/1), riboflavin (0.1 mg/1), ascorbic acid (50 mg/l), biotin (0.01 mg/1), lysine (12.5 mg/1), asparagine (16 mg/1), glycine (8 mg/1) and 0.5 mg 2,4-Dichloro- phenoxyacetic acid and 10 mg benzylaminopurine (BAP). The growch regulators 2,4-D and BAP, and vitamins pyridoxine-HCl1 and ascorbic acid were found to be required in the induction of callus formation in white lauan, Amino acids asparagine, glycine and lysine appear to be necessary in the maintenance of the callus. Sprout explants formed better callus than shoot explants. Cultures incubated in the dark formed callus better than those incubated in the light.

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Holdings ( 1 )
Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	634.9 L31 1983 (Browse shelf(Opens below))		Link to resource	Room use only	T-1341		00002271

Thesis (M.S. - - Forestry) University of the Philippines, College, Laguna.

Includes bibliographical references.

LAPITAN, PORTIA G. University of the Philippines at Los Bafios, May, 1983. Nutritional Requirements of White Lauan (Shorea _contorta Vid.) for Callus InitiaTion. Major Professor: Dr. M. U. Garcia

Eleven culture media were tested to induce callus initiation on sprout and shoot explants of white lauan (Shorea contorta Vid.). Only 3 media were found to induce callus initiation and the best was medium IX, the basal medium of Murashige and Skoog supplemented with myoinositol (100 mg/1), nicotinic acid (0.5 mg/1), thiamine-HC1 (0.1 mg/1), pyridoxine-HC1 (0.1 mg/l), choline chloride (10 mg/1), calcium pantothenate (0.1 mg/1), riboflavin (0.1 mg/1), ascorbic acid (50 mg/l), biotin (0.01 mg/1), lysine (12.5 mg/1), asparagine (16 mg/1), glycine (8 mg/1) and 0.5 mg 2,4-Dichloro- phenoxyacetic acid and 10 mg benzylaminopurine (BAP).

The growch regulators 2,4-D and BAP, and vitamins pyridoxine-HCl1 and ascorbic acid were found to be required in the induction of callus formation in white lauan, Amino acids asparagine, glycine and lysine appear to be necessary in the maintenance of the callus. Sprout explants formed better callus than shoot explants. Cultures incubated in the dark formed callus better than those incubated in the light.

Submitted to the University Library 01/07/1994 T-1341