Design and development of microcontroller-based tissue culture growth cabinet / by Camille Joy V. Capupus and Quennie J. Rosarda.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite: Cavite State University - Main Campus, 2005.Description: xviii, 84 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 620.0042  C17 2005
Online resources: Production credits:
  • College of Engineering and Information Technology (CEIT)
Summary: CAPUPUS, CAMILLE JOY V. and QUENNIE J. ROSARDA. Design and Development of Microcontroller-Based Tissue Culture Growth Cabinet. Undergraduate Design Project. Bachelor of Science in Computer Engineering. Cavite State University, Indang, Cavite. April 2005. Advisers: Mr. Bienvenido C. Sarmiento Jr. and Engr. Jaime Q. Dilidili The main objective of the study was to design and develop a microcontroller-based tissue culture growth cabinet. Specifically, it aimed to: construct a tissue culture growth cabinet; create a software that will interface the components of the culture cabinet using assembly language; conduct test runs for the culture cabinet in terms of providing the desired temperature, light intensity and photoperiod; and conduct cost computation of the system. Hence, the flow is on the matter of controlling the environment inside the tissue culture growth cabinet through the system that was constructed. The tissue culture growth cabinet was composed of two major components, the controller unit and the tissue culture growth cabinet itself. The controller unit being the electronic and computer part of the system controls the operation. It served as the brain of the system because it tells when the execution of the operation is allowed or when it is not. It was composed of integrated circuits aid other component that were connected through data and address buses. The tissue culture growth cabinet was the body of the system. It was composed of a detachable cabinet that has three-layer perforated trays that can accommodate 255 pieces catsup bottles, 14 pieces of 48" 40W cool-white fluorescent lamps that served as light source, four ventilating fans which were used to circulate the air inside the cabinet, and a heater which supplied as the heat requirement. The software was developed using assembly language. The software controlled the operation of the machine capable of instructing the whole operation such as supplying the required temperature, and light intensity, and enabling to provide the length of day and night ambiance needed. It was divided into subroutines thus no need to create a long single continuous one. The system was evaluated by comparing the data gathered between the microcontroller-based tissue culture growth cabinet and the existing tissue culture growth cabinet used in the university research laboratory. Survival rate of 83.33 percent was obtained using the developed system while 73.33 percent survival rate using the conventional means. The average room temperatures observed at 8:30 AM, 12:30 NN, and 4:30 PM were 26.3 °C, 27.8 °C, and 29.4°C respectively while the average operating temperatures were 28.5 °C, 28.8°C, and 28.7°C respectively. Also, the recommended 8/16 photoperiod was used in the culture of makapuno embryo. The performance of the improved system was compared to the conventional system. The microcontroller-based tissue culture growth cabinet hastened the growth and germination of the makapuno embryo.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 620.0042 C17 2005 (Browse shelf(Opens below)) Link to resource Room use only DP-147 00001221

Design Project (BS Computer Engineering) Cavite State University.

Includes bibliographical references.

College of Engineering and Information Technology (CEIT)

CAPUPUS, CAMILLE JOY V. and QUENNIE J. ROSARDA. Design and Development of Microcontroller-Based Tissue Culture Growth Cabinet. Undergraduate Design Project. Bachelor of Science in Computer Engineering. Cavite State University, Indang, Cavite. April 2005. Advisers: Mr. Bienvenido C. Sarmiento Jr. and Engr. Jaime Q. Dilidili
The main objective of the study was to design and develop a microcontroller-based tissue culture growth cabinet. Specifically, it aimed to: construct a tissue culture growth cabinet; create a software that will interface the components of the culture cabinet using assembly language; conduct test runs for the culture cabinet in terms of providing the desired temperature, light intensity and photoperiod; and conduct cost computation of the system. Hence, the flow is on the matter of controlling the environment inside the tissue culture growth cabinet through the system that was constructed. The tissue culture growth cabinet was composed of two major components, the controller unit and the tissue culture growth cabinet itself. The controller unit being the electronic and computer part of the system controls the operation. It served as the brain of the system because it tells when the execution of the operation is allowed or when it is not. It was composed of integrated circuits aid other component that were connected through data and address buses. The tissue culture growth cabinet was the body of the system. It was composed of a detachable cabinet that has three-layer perforated trays that can accommodate 255 pieces catsup bottles, 14 pieces of 48" 40W cool-white fluorescent lamps that served as light source, four ventilating fans which were used to circulate the air inside the cabinet, and a heater which supplied as the heat requirement. The software was developed using assembly language. The software controlled the operation of the machine capable of instructing the whole operation such as supplying the required temperature, and light intensity, and enabling to provide the length of day and night ambiance needed. It was divided into subroutines thus no need to create a long single continuous one. The system was evaluated by comparing the data gathered between the microcontroller-based tissue culture growth cabinet and the existing tissue culture growth cabinet used in the university research laboratory. Survival rate of 83.33 percent was obtained using the developed system while 73.33 percent survival rate using the conventional means. The average room temperatures observed at 8:30 AM, 12:30 NN, and 4:30 PM were 26.3 °C, 27.8 °C, and 29.4°C respectively while the average operating temperatures were 28.5 °C, 28.8°C, and 28.7°C respectively. Also, the recommended 8/16 photoperiod was used in the culture of makapuno embryo. The performance of the improved system was compared to the conventional system. The microcontroller-based tissue culture growth cabinet hastened the growth and germination of the makapuno embryo.

Submitted to the University Library 04/12/2005 DP-147

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