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Identity and biosorptive capability of metal-resistant bacteria isolated from Bayto River in Sta. Cruz, Zambales / John Rex N. Baldomero.

By:

Baldomero, John Rex N [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2018.Description: xviii , 78 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

540 B18 2018

Online resources:

View Abstract

Production credits:

College of Arts and Sciences (CAS), Department of Biological Sciences.

Abstract: BALDOMERO, JOHN REX N. Identity and Biosorptive Capability of Metal-resistant Bacteria Isolated from Bayto river in Sta. Cruz, Zambates. Undergraduate Thesis. Bachelor of Science in Biology. College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2018. Adviser: Dr. Yolanda A. Hagan Heavy metal contamination of water systems is a growing environmental concern worldwide; conventional techniques in the removal of heavy metals in polluted ecological niches are costly and may produce secondary waste products. On the other hand, bioremediation, specifically biosorption, offers a more potent and cost-effective solution to this problem. The study intended to isolate and identify metal-resistant bacteria with biosorptive capability. A total of 139 Nickel-resistant isolates were obtained from the water samples collected from Bayto River. Sixteen isolates were able to tolerate the highest concentration of nickel and were subjected to multimetal resistance assay. Out of the 16 most nickel-resistant isolates, only four were able to tolerate 7,500 ppm Cu, and 10,000 ppm Cr and Pb. These isolates namely S2Q1, 5112, S3Z1, and S2P1 were considered as the most promising isolates and were subjected to identification by the 16S RNA sequencing, bio sorption assay, and plasmid isolation. S2Q1 and and S112 were identified as Stenotrophomonas rnaltophilia while S3Z1 and S2P1 as Bacillus cereus. Moreover, S1I2 exhibited the highest percent bio sorption (92.27%), followed by S3Z1 (91.67%), then S2Q1 (91. 36), and S2P1 (89.78%). Plasmid isolation was attempted from the most promising isolates using alkaline lysis method. However, after several attempts, no sign of the presence of plasmids was observed as confirmed by the agarose gel electrophoresis profiles of these isolates.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	540 B18 2018 (Browse shelf(Opens below))		Room use only	T-7725		00076592

Thesis (Bachelor of Science in Biology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences.

BALDOMERO, JOHN REX N. Identity and Biosorptive Capability of Metal-resistant Bacteria Isolated from Bayto river in Sta. Cruz, Zambates. Undergraduate Thesis. Bachelor of Science in Biology. College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2018. Adviser: Dr. Yolanda A. Hagan
Heavy metal contamination of water systems is a growing environmental concern worldwide; conventional techniques in the removal of heavy metals in polluted ecological niches are costly and may produce secondary waste products. On the other hand, bioremediation, specifically biosorption, offers a more potent and cost-effective solution to this problem. The study intended to isolate and identify metal-resistant bacteria with biosorptive capability. A total of 139 Nickel-resistant isolates were obtained from the water samples collected from Bayto River. Sixteen isolates were able to tolerate the highest concentration of nickel and were subjected to multimetal resistance assay. Out of the 16 most nickel-resistant isolates, only four were able to tolerate 7,500 ppm Cu, and 10,000 ppm Cr and Pb. These isolates namely S2Q1, 5112, S3Z1, and S2P1 were considered as the most promising isolates and were subjected to identification by the 16S RNA sequencing, bio sorption assay, and plasmid isolation.
S2Q1 and and S112 were identified as Stenotrophomonas rnaltophilia while S3Z1 and S2P1 as Bacillus cereus. Moreover, S1I2 exhibited the highest percent bio sorption (92.27%), followed by S3Z1 (91.67%), then S2Q1 (91. 36), and S2P1 (89.78%). Plasmid isolation was attempted from the most promising isolates using alkaline lysis method. However, after several attempts, no sign of the presence of plasmids was observed as confirmed by the agarose gel electrophoresis profiles of these isolates.

Submitted to the University Library December 05, 2018 T-7725