In vitro amoebicidal activity if yerba buena (Mentha arvensis) leaf extract against entamoeba histolytica / by Reilene Ann M. Ambojica and Anne Magdalene A. Cadiliman.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2018.Description: xi, 79 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 633.82  Am1 2018
Online resources: Production credits:
  • College of Nursing (CON), Department of Medical Technology
Abstract: AMBOJIA, REILENE ANN, CADILIMAN, ANNE MAGDALENE, In Vitro Assoebicidal Activity of Yerba Buena (Mentha arvensis) Leaf Extract Against Eitamoeba histolytica Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, December 2017, Adviser: Lenila A. De Vera, RN, MPH, MAN, Co-Adviser: Jo Hanes D. Contemprato, RMT. This study, "In Vitro Amoebicidal Activity of Yerba Buena (Mentha arvensis) Leaf Extract Against Entamoeba histolytica", was conducted at the Department of Medical Technology, College of Nursing from July 2017 to September 2017. The study generally aimed to determine the potential of Yerba Buena (Mentha arvensis) leaf extract as an alternative amoebicidal agent against Entamoeba histolytica; determine which concentration of extract will yield the most significant antiamoebic effect; and determine the duration of action of Yerba Buena (Mentha arvensis) leaf extract. Experimental research approach was used in this study to test the effect of Yerba Buena (Mentha arvensis) leaf extract against Entamoeba histolytica. Stool sample positive for amoebiasis was collected from San Lorenzo Ruiz Hospital. Yerba Buena leaves were collected from Amadeo, Cavite FITS Center. The leaves were extracted by ethanol with the Soxhlet apparatus. The stool sample was inoculated and cultured in Locke-Egg medium. After incubation, the samples were viewed under the microscope to detect the presence and growth of Entamoeba histolytica. The 50 percent concentration of Yerba Buena (Mentha arvensis) leaf extract had the highest mean of dead cysts with the value of 6.83 while the 25 percent concentration had the lowest mean of dead cysts with the value of 4.12. Among the different concentrations of extract, the 50 percent concentration had comparable results from the positive control, thus, it was the concentration that yield the most significant antiamoebic effect. Since the significance level of Yerba Buena (Mentha arvensis) leaf extract is less than 0.05 in the 12th and 24thhr, the duration of action of the extract was 24 hrs. Therefore, Yerba Buena (Mentha arvensis) leaf extract had an antiamoebic effect against Entamoeba hisiolyiieu. Keywords: Yerba Buena, Amoebicidal activity, Entamoeba histolytica, Ethanol
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 633.82 Am1 2018 (Browse shelf(Opens below)) Link to resource Room use only RS-830 00077288

Research Study (Bachelor of Science in Medical Technology) Cavite State University.

Includes bibliographical references.

College of Nursing (CON), Department of Medical Technology

AMBOJIA, REILENE ANN, CADILIMAN, ANNE MAGDALENE, In Vitro Assoebicidal Activity of Yerba Buena (Mentha arvensis) Leaf Extract Against Eitamoeba histolytica Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, December 2017, Adviser: Lenila A. De Vera, RN, MPH, MAN, Co-Adviser: Jo Hanes D. Contemprato, RMT.
This study, "In Vitro Amoebicidal Activity of Yerba Buena (Mentha arvensis) Leaf Extract Against Entamoeba histolytica", was conducted at the Department of Medical Technology, College of Nursing from July 2017 to September 2017. The study generally aimed to determine the potential of Yerba Buena (Mentha arvensis) leaf extract as an alternative amoebicidal agent against Entamoeba histolytica; determine which concentration of extract will yield the most significant antiamoebic effect; and determine the duration of action of Yerba Buena (Mentha arvensis) leaf extract. Experimental research approach was used in this study to test the effect of Yerba Buena (Mentha arvensis) leaf extract against Entamoeba histolytica. Stool sample positive for amoebiasis was collected from San Lorenzo Ruiz Hospital. Yerba Buena leaves were collected from Amadeo, Cavite FITS Center. The leaves were extracted by ethanol with the Soxhlet apparatus. The stool sample was inoculated and cultured in Locke-Egg medium. After incubation, the samples were viewed under the microscope to detect the presence and growth of Entamoeba histolytica. The 50 percent concentration of Yerba Buena (Mentha arvensis) leaf extract had the highest mean of dead cysts with the value of 6.83 while the 25 percent concentration had the lowest mean of dead cysts with the value of 4.12. Among the different concentrations of extract, the 50 percent concentration had comparable results from the positive control, thus, it was the concentration that yield the most significant antiamoebic effect. Since the significance level of Yerba Buena (Mentha arvensis) leaf extract is less than 0.05 in the 12th and 24thhr, the duration of action of the extract was 24 hrs. Therefore, Yerba Buena (Mentha arvensis) leaf extract had an antiamoebic effect against Entamoeba hisiolyiieu.
Keywords:
Yerba Buena, Amoebicidal activity, Entamoeba histolytica, Ethanol

Submitted to the University Library July 16, 2018 RS-830

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