Detection of subclinical mastitis in dairy buffaloes (babalus bubalis) from selected farms from Cavite / by Kim Casey Dahilog Solitario.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xii, 51 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.2142  So4 2017
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: SOLITARIO, KIM CASEY D. Detection of subclinical mastitis in dairy buffaloes (Bubalus bubalis) in selected farms in Cavite. Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. July 2017. Adviser: Chester Joshua V. Saldafia, DVM, MSc. A study was conducted to detect the presence of subclinical mastitis in raw milk of dairy buffaloes in Cavite. A total of 59 milk samples from dairy buffalo backyard farms in General Trias, Cavite were enrolled in the study. Milk samples were collected and processed immediately for California Mastitis Test. Further tests were conducted in the laboratory to determine the Somatic Cell Count (SCC) using Fossomatic™ FC and the Total Viable Count (TVC) of milk samples by serially diluting the milk up to 10’ using normal saline solution as diluent, plated onto Nutrient Agar plates and incubated at various temperatures. Results of the study revealed that 15.25% (9/59) of the total dairy buffaloes were positive for subclinical mastitis. Seven out of 20 (35%) samples from Brgy. Pasong Camichille I and Two out of 14 (14%) samples from Brgy. Santiago were positive for subclinical mastitis. The Total Viable Count (TVC) of fresh buffalo milks ranged from 1.12 x 10° to 2.79 x 10°. The mean viable count at 37°C incubation is 7.5 x 10° cfu/mL. This is higher compared with the viable counts of 4.7 x 10° cfu/mL at 25 °C although no statistical difference was found. No growth was observed at 5 °C and 59 °C. Furthermore, the presence of subclinical mastitis was statistically associated with the total viable count. The somatic cell count (SCC) of fresh buffalo milks from sample farms in Cavite ranges from 1.4 x 10* to 8.8 x 10° with a mean of 1.5 x 10° cells/ml. Moreover, the Total . Viable Count (TVC) and Somatic Cell Count (SCC) have sensitivity of 89% and 100% and specificity of 96% and 100% respectively. This means that if mastitis is present, there is an 89% chance that TVC and 100% chance that SCC will detect it. The Somatic Cell Count (SCC) is a dependable test to diagnose subclinical mastitis in buffaloes like the California Mastitis Test (CMT) that are commonly used in dairy farms. This study revealed that the subclinical mastitis is prevalent in milking buffaloes in Cavite.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.2142 So4 2017 (Browse shelf(Opens below)) Link to resource Room use only T-7097 00011683

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

SOLITARIO, KIM CASEY D. Detection of subclinical mastitis in dairy buffaloes (Bubalus bubalis) in selected farms in Cavite. Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. July 2017. Adviser: Chester Joshua V. Saldafia, DVM, MSc.

A study was conducted to detect the presence of subclinical mastitis in raw milk of dairy buffaloes in Cavite. A total of 59 milk samples from dairy buffalo backyard farms in General Trias, Cavite were enrolled in the study. Milk samples were collected and processed immediately for California Mastitis Test. Further tests were conducted in the laboratory to determine the Somatic Cell Count (SCC) using Fossomatic™ FC and the Total Viable Count (TVC) of milk samples by serially diluting the milk up to 10’ using normal saline solution as diluent, plated onto Nutrient Agar plates and incubated at various
temperatures.

Results of the study revealed that 15.25% (9/59) of the total dairy buffaloes were positive for subclinical mastitis. Seven out of 20 (35%) samples from Brgy. Pasong Camichille I and Two out of 14 (14%) samples from Brgy. Santiago were positive for subclinical mastitis. The Total Viable Count (TVC) of fresh buffalo milks ranged from
1.12 x 10° to 2.79 x 10°. The mean viable count at 37°C incubation is 7.5 x 10° cfu/mL. This is higher compared with the viable counts of 4.7 x 10° cfu/mL at 25 °C although no statistical difference was found. No growth was observed at 5 °C and 59 °C. Furthermore, the presence of subclinical mastitis was statistically associated with the total viable count.

The somatic cell count (SCC) of fresh buffalo milks from sample farms in Cavite ranges from 1.4 x 10* to 8.8 x 10° with a mean of 1.5 x 10° cells/ml. Moreover, the Total . Viable Count (TVC) and Somatic Cell Count (SCC) have sensitivity of 89% and 100% and specificity of 96% and 100% respectively. This means that if mastitis is present, there is an 89% chance that TVC and 100% chance that SCC will detect it. The Somatic Cell Count (SCC) is a dependable test to diagnose subclinical mastitis in buffaloes like the California Mastitis Test (CMT) that are commonly used in dairy farms.

This study revealed that the subclinical mastitis is prevalent in milking buffaloes in Cavite.

Submitted copy to the University Library. 09/20/2017 T-7097

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