Screening for the anthelmintic activity of Actinomycetes isolates against Ascaris suis from soil samples in selected areas of Indang, Cavite / by Gianne D. Lontoc, Paulina Alexandra C. Nunga and Margarete D. Santos.

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

LONTOC, GIANNE D., NUNGA, PAULINA ALEXANDRA C., AND SANTOS, MARGARETE D. Screening for the Anthelmintic activity of Actinomycetes isolates against Ascaris suis from soil samples in selected areas of Indang, Cavita Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University — Indang, Cavite, November 2016. Adviser: Chester Joshua V. Saldafia, DVM, MS and Karen Krista M. Escobar, RMT, MSMT.
The study was conducted at the College of Nursing and College of Veterinary Medicine, Cavite State University, Indang, Cavite from June 2016 to November 2016. The study aimed to isolate and characterize the features of Actinomycetes morphologically and physiologically; also to determine the effects of Actinomycetes isolates against the ova and adult worm of Ascaris suis and determine which among the Actinomycetes isolates are capable of producing 50 percent and 100 percent mortality against ova and adult worm ofAscaris suis.
Soil samples were collected, dried, processed to water and was inoculated onto yeast malt agar (YMA) plates with nystatin and incubated for 3 to 7 days at room temperature. The isolates were observed daily. The cultural, motphological and physiologic characteristics were recorded to determine the identity of the Actinomycetes isolates. The isolates were inoculated on trypticase soy broth and cultured for 7 days. For the ovocidal activity, mature ova was collected and placed in microtiter plates with extract. On the other hand, adult worms were submerged into cultured trypticase soy broth treatment and observed for 48 hours. All treatments were performed in triplicate.
The mortality were recorded and compared.
Results revealed that majority of the isolated Actinomycetes were from Bancod
(60%) followed by Kaytapos (33%) and least from Buna Cerca (7%). These isolates were vii
gathered from varying soil types ranging from loam to sandy and the majority were from loam soil (60%) but no statistical relationship was found between the soil type and the number of Actinomycetes isolated. Actinomycetes isolates grew well in room temperature producing small (2-3mm) to large (more than 6mm), dry, scaly to flaky, irregular to flat raised colonies embedded on the medium. All colonies were opaque with smooth surface but later developed into powdery or velvety. Based on color, 87 percent produced white while 13 percent produced brown colonies. Majority of the growth were observed within 3 days. Microscopically, four out of fifteen (27%) isolates produced spore chain and special structures were not observed among the isolates. Moreover, isolates were able to ferment (67%) and oxidize (33%) different carbon sources, hydrolyze starch (93%) and urea (13%), liquefy gelatin (47%) and able to produce 1-12s (53%).
Results of the Anthelmintic activity showed that the Actinomycetes isolates were capable of producing mortality to Ascaris suis ova and adult worms. For the ovocidal activity, all of the isolates were able to inhibit the development of ova but four isolates inhibit the development of more than 50 percent ova. For the adulticidal activity, all isolates except one produced 100 percent mortality at 48 hour exposure time, paralyzing and killing all of the Ascaris suis adult and 50 percent mortality was observed during 24 hour post exposure. The results showed that the anthelmintic producing Actinomycetes isolated from soil samples in Indang, Cavite were good instance for the production of bioactive secondary metabolites that can be used for medicinal purposes in the future and further studies.

Submitted copy to the University Library. 05/02/2017 T-6602