Utilization of garlic (Allum sativum) extract against common fungus (helminthosporium sp.) affecting the in vitro grower makapuno seedlings / by Von Eisen G. Alcazar, Katherine Aira N. Delos Santos, and Michael Raphael D. Ikan.
Material type:
TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2006.Description: xv, 38 pages : illustrations ; 28 cmContent type: - text
- unmediated
- volume
- 635.26 Al1 2006
- Science High School, College of Education (CED)
| Item type | Current library | Collection | Call number | Materials specified | URL | Status | Notes | Date due | Barcode |
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 635.26 Al1 2006 (Browse shelf(Opens below)) | Link to resource | Room use only | R-499 | 00000968 |
Research Study (Applied Research IV - - General Science Curriculum) Cavite State University.
Includes bibliographical references.
Science High School, College of Education (CED)
ALCAZAR, VON EISEN G., DELOS SANTOS, KATHRINE AIRA N.,
IKAN, MICHAEL RAPHAEL D, Applied Research 111 (General Science Curriculum) Cavite State University Science High School, Indang, Cavite, March 2006. "Utilization of Garlic (Allium Sativum) Extracts Against Common Fungus (Helminthosporium sp.) Affecting the in Vitro Grown Makapuno Seedlings." Adviser: Dr. Amornita C. Sanchez.
The use of garlic (Allium Sativum) extracts against common fungus (Helminthosporium sp.) affecting in vitro grown makapuno seedlings was evaluated at the Makapuno Culture Laboratory and Crop Protection Laboratory of the Department of
Crop Sciences at the College of Agriculture, Forestry, Environment and• Natural Resources (CAFENR), Cavite State University, Indang, Cavite. The study aimed to identify the most effective method of using garlic extract against Helminthosporium sp.
and to determine the efficacy of garlic extracts against Helminthosporium sp. in in vitro cultured makapuno seedlings.
The different treatments evaluated were: Treatment 1 (T 1) — dipping the whole plant in pure garlic extract before planting in inoculated Y3 media, Treatment 2 (T2) — adding 1 ml of pure garlic extract in a fungus-inoculated Y3 media before planting into it the makapuno seedlings, and Treatment 3 (T3) — combination of Treatment 1 (T 1) and Treatment 2 (T2). The occurrence and the degree of infection by fungus in makapuno seedlings and media were observed two, four and six days after the culture. The best treatment to control fungal infection was the addition of I ml- of pure garlic extract in a fungus-inoculated media before makapuno embryos are cultured.
There was no sign of fungal infection in all treatments in two days after application of garlic extract. All the Y3 media were found to be clear with the garlic extracts settled at the bottom of the test tubes. Four days after the application of garlic extracts, all the plants still had no sign of fungal infection. However, the seedlings dipped in pure garlic extracts (T 1) were found to have brownish roots. The same effects were noticed on the seedlings six days after garlic application. The seedlings in T2 (garlic extract applied in fungus- inoculated media) were found to be normal with no visible side effects six days after application. The seedlings from T3 (combination of Tl and T2) were normal with no visible signs of fungal infection but the roots became brownish on the fourth day and which advanced into more intense brown color after six days.
Despite browning of roots, all the treatments were found to be very effective against the Helminthosporium sp. since no fungal infection was observed from the makapuno seedlings. The results did not affect the life of the plants but just delayed the formation of more roots.
Submitted to the University Library R-499