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Production of fungal cellulase using sugarcane bagasse as carbon susbstrate / by Laureano L. Bondoc, Jr.

By:

Bondoc, Laureano L., Jr [author]

Material type: Text

TextLanguage: English Publication details: Los Baños, Laguna : 1982. Cavite State University- Main Campus,Description: 108 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

633.61 B64 1982

Online resources:

Click here to view thesis abstract and table of contents

Abstract: BONDOC, LAUREANO, JR. University of the Philippines at Los Bafios, October, 1982, Production of Fungal Cellulase Using Sugarcane Bagasse as Carbon Substrate, Major Professor: Dr, Ernesto J, del Rosario Cellulase production was optimized with respect to incubation time and substrate composition for Trichoderma reesei NRRL 11485, Trichoderma viride QM 9414, and Penicillium sp. 86 (a local isolate) grown on sugarcane bagasse in shake flask cultures at room temperature (ca, 30°C), Of the three fungal strains tested, Penicillium sp, 86 elaborated a potent cellulase exhibiting the highest activities when grown on 5% untreated bagasse (80 mesh) supplemented with urea and (NH4),50,, The cellulase activities are comparable to those recently reported for Trichoderma longibrachiatum and Aspergillus terreus GN 1, Using the optimal substrate composition, cellulase production by Penicillium sp. 86 was further optimised with respect to solids level content and pH using either untreated bagasse or alkali~treated bagasse in batch culture using an airlift fermenter with aeration at lvvm at room temperature (ca, 30°C), When the organism was cultured on 2.0% untreated bagasse with pH controlled at 4, 5, maximum enzyme activities were obtained after 48 hours fermentation, namely, Filter Paper (FP) Volumetric Activity (VA), 0.0883 IU/ml; FP Specific Activity (SA), 0,1193 IU/mg P; Carbo-xymethy! cellulose (CMC) VA, 0.8898 IU/ml; and CMCSA, 2.0086 1U/mg P.

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Holdings ( 1 )
Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	633.61 B64 1982 (Browse shelf(Opens below))		Link to resource	Room use only	T-1335		00002267

Thesis (M.S. - - Agricultural Chemistry) University of the Philippines, College, Laguna.

Includes bibliographical references.

BONDOC, LAUREANO, JR. University of the Philippines at Los Bafios, October, 1982, Production of Fungal Cellulase Using Sugarcane Bagasse as Carbon Substrate, Major Professor: Dr, Ernesto J, del Rosario

Cellulase production was optimized with respect to incubation time and substrate composition for Trichoderma reesei NRRL 11485, Trichoderma viride QM 9414, and Penicillium sp. 86 (a local isolate) grown on sugarcane bagasse in shake flask cultures at room temperature (ca, 30°C), Of the three fungal strains tested, Penicillium sp, 86 elaborated a potent cellulase exhibiting the highest activities when grown on 5% untreated bagasse (80 mesh) supplemented with urea and (NH4),50,,

The cellulase activities are comparable to those recently reported for Trichoderma longibrachiatum and Aspergillus terreus GN 1, Using the optimal substrate composition, cellulase production by Penicillium sp. 86 was further optimised with respect to solids level content and pH using either untreated bagasse or alkali~treated bagasse in batch culture using an airlift fermenter with aeration at lvvm at room temperature (ca, 30°C), When the organism was cultured on 2.0% untreated bagasse with pH controlled at 4, 5, maximum enzyme activities were obtained after 48 hours fermentation, namely, Filter Paper (FP) Volumetric Activity (VA), 0.0883
IU/ml; FP Specific Activity (SA), 0,1193 IU/mg P; Carbo-xymethy! cellulose (CMC) VA, 0.8898 IU/ml; and CMCSA, 2.0086 1U/mg P.

Submitted to the University Library 01/07/1994 T-1335