Nutritional requirements of white lauan (Shore contorta Vid) for callus initiation / by Portia Gamboa Lapitan
Material type: TextLanguage: English Publication details: Los Baños, Laguna : 1983. Cavite State University- Main Campus,Description: 76 pages : illustrations ; 28 cmContent type:- text
- unmediated
- volume
- 634.9 L31 1983
Item type | Current library | Collection | Call number | Materials specified | URL | Status | Notes | Date due | Barcode |
---|---|---|---|---|---|---|---|---|---|
Theses / Manuscripts | Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 634.9 L31 1983 (Browse shelf(Opens below)) | Link to resource | Room use only | T-1341 | 00002271 |
Thesis (M.S. - - Forestry) University of the Philippines, College, Laguna.
Includes bibliographical references.
LAPITAN, PORTIA G. University of the Philippines at Los Bafios, May, 1983. Nutritional Requirements of White Lauan (Shorea _contorta Vid.) for Callus InitiaTion. Major Professor: Dr. M. U. Garcia
Eleven culture media were tested to induce callus initiation on sprout and shoot explants of white lauan (Shorea contorta Vid.). Only 3 media were found to induce callus initiation and the best was medium IX, the basal medium of Murashige and Skoog supplemented with myoinositol (100 mg/1), nicotinic acid (0.5 mg/1), thiamine-HC1 (0.1 mg/1), pyridoxine-HC1 (0.1 mg/l), choline chloride (10 mg/1), calcium pantothenate (0.1 mg/1), riboflavin (0.1 mg/1), ascorbic acid (50 mg/l), biotin (0.01 mg/1), lysine (12.5 mg/1), asparagine (16 mg/1), glycine (8 mg/1) and 0.5 mg 2,4-Dichloro- phenoxyacetic acid and 10 mg benzylaminopurine (BAP).
The growch regulators 2,4-D and BAP, and vitamins pyridoxine-HCl1 and ascorbic acid were found to be required in the induction of callus formation in white lauan, Amino acids asparagine, glycine and lysine appear to be necessary in the maintenance of the callus. Sprout explants formed better callus than shoot explants. Cultures incubated in the dark formed callus better than those incubated in the light.
Submitted to the University Library 01/07/1994 T-1341