Frozen buck semen processed in straws and ampules and its fertilizing capacity using different spermatozoan concentration / by Cayetano S. Pomares.

By: Material type: TextTextLanguage: English Publication details: Los Baños, Laguna, 1983. Cavite State University- Main Campus,Description: 101 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.39  P77 1983
Online resources: Production credits:
  • College of Agriculture, Food, Environment and Natural Resources (CAFENR)
Abstract: POMARES, CAYETANO CARINO. University of the Philippines at Los Banos, September 1983. Frozen Buck Semen Processed in Straws and Ampules and Its Fertilizing CapacityUsing Different Spermatozoan Concentrations. Major Professor: Dr. Edwin G. Wagelie A study on deep-freezing of buck semen using different packaging methods and concentrations was conducted at the University of the Philippines at Los Banos, College, Laguna to determine its fertility and viability on artificial breeding of goats in the Philippines. Semen collected from 3 to 4-year old Anglo-Nubian bucks was assessed for its general appearance, morphology, spermatozoa concentration, volume, and motility. The buck semen appeared creamy and showed thick viscosity or consistency. Morphological examinations showed 75, 40% normal and 24.06% abnormal spermatozoa. Abnormalities were mostly observed at the tail portion. Spermatozoa concentrations of the three (3) bucks are similar. The volume of ejaculates ranged from 0.3 to 2.0 ml with a grand mean of 0.9 ml for 7 collections. Buck semen extended with Tris-egg-yolk raffinose and frozen in liquid nitrogen did not show significant difference in motility before and after freezing, Semen frozen at -196°C and packaged in ampules and straws for 18 months significantly differed (P< 0.01) in its post-thawing motility. The ampule-packaged 60 million (M) spermatozoa/ml had higher (PK 0.01) post-thaw motility and sperm revival than the 80 and 100M/m1. On the other hand, semen packaged in straw at 60 and 80M spermatozoa/0, 5 ml showed inferior post-thaw motility and revival, However semen motility at 100M spermatozoa/0.5 ml in straw was comparable with 1001VI spermatozoa/ ml in ampules up to nine months of storage. Beyond 9 months, motility declined in the straw-packaged semen but not in the semen stored in ampules, The fertility test on semen packaged in ampules and straws with 60, 80, and 100M concentration frozen in liquid nitrogen revealed a considerably low conception rate (CR), Highest CR of 33.33% was observed with semen in ampules at 100M spermatozoa/ml followed by 20.00 and 0% at 80 and 60M spermatozoa/m1 respectively. Five twins and single kids resulted from eight does with an average gestation period of 146.5 days; Pregnancy did not occur in straw-packaged semen at all concentration levels.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Materials specified URL Status Notes Date due Barcode
Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.39 P77 1983 (Browse shelf(Opens below)) Link to resource Room use only T-1555 00006237

Thesis (M.S.--Animal Science) University of the Philippines

Includes bibliographical references.

College of Agriculture, Food, Environment and Natural Resources (CAFENR)

POMARES, CAYETANO CARINO. University of the Philippines at Los Banos, September 1983. Frozen Buck Semen Processed in Straws and Ampules and Its Fertilizing CapacityUsing Different Spermatozoan Concentrations.
Major Professor: Dr. Edwin G. Wagelie
A study on deep-freezing of buck semen using different packaging methods and concentrations was conducted at the University of the Philippines at Los Banos, College, Laguna to determine its fertility and viability on artificial breeding of goats in the Philippines.
Semen collected from 3 to 4-year old Anglo-Nubian bucks was assessed for its general appearance, morphology, spermatozoa concentration, volume, and motility. The buck semen appeared creamy and showed thick viscosity or consistency. Morphological examinations showed 75, 40% normal and 24.06% abnormal spermatozoa. Abnormalities were mostly observed at the tail portion. Spermatozoa concentrations of the three (3) bucks are similar. The volume of ejaculates ranged from 0.3 to 2.0 ml with a grand mean of 0.9 ml for 7 collections.
Buck semen extended with Tris-egg-yolk raffinose and frozen in liquid nitrogen did not show significant difference in motility before and after freezing, Semen frozen at -196°C and packaged in ampules and straws for 18 months significantly differed (P< 0.01) in its post-thawing motility.
The ampule-packaged 60 million (M) spermatozoa/ml had higher (PK 0.01) post-thaw motility and sperm revival than the 80 and 100M/m1. On the other hand, semen packaged in straw at 60 and 80M spermatozoa/0, 5 ml showed inferior post-thaw motility and revival, However semen motility at 100M spermatozoa/0.5 ml in straw was comparable with 1001VI spermatozoa/ ml in ampules up to nine months of storage. Beyond 9 months, motility declined in the straw-packaged semen but not in the semen stored in ampules,
The fertility test on semen packaged in ampules and straws with 60, 80, and 100M concentration frozen in liquid nitrogen revealed a considerably low conception rate (CR), Highest CR of 33.33% was observed with semen in ampules at 100M spermatozoa/ml followed by 20.00 and 0% at 80 and 60M spermatozoa/m1 respectively. Five twins and single kids resulted from eight does with an average gestation period of 146.5 days; Pregnancy did not occur in straw-packaged semen at all concentration levels.

Submitted to the University Library 07/18/2007 T-1555

Copyright © 2023. Cavite State University | Koha 23.05