Antimicrobial activity of koro-korosan (Chloris barbata (Linn.)) leaf and root extract / by Joanna Marie B. Dispulo, Karyssa Janelle R. Lopez and Sarah Mae B. Muralla.
Material type:
TextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xv, 78 pages : 28 cm. illustrations ; Content type: - text
- unmediated
- volume
- 615.8 D63 2015
- College of Nursing (CON)
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 615.8 D63 2015 (Browse shelf(Opens below)) | Link to resource | Room use only | T-6142 | 00010177 |
Thesis (BS Medical Technology) Cavite State University
Includes bibliographical references.
College of Nursing (CON)
Dispulo, Joana Marie B., Lopez, Karyssa JaneDe R., Muralla, Sarah Mae B., ANTIMICROBIAL ACTIVITY OF KORO-KOROSAN (Chloris barbata (Linn.)) LEAF AND ROOT EXTRACT. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, November 2015. Adviser: Mrs. Adelaida Sangalang RMT,PhD
The study was conducted in Cavite State University College of Nursing Depatment of Medical Technology from September 2014 to January 2015. The study aimed to determine the antimicrobial activity of leaf and root extract of Koro-korosan (Chloris barbata (Linn.)) against Enterobacter aerogenes, Serratia marcescens, Fusarium oxysporum and Aspergillus niger. Specifically, it aims to identify which part of Koro-korosan (Chloris barbata (Linn.)) has been effective in inhibiting the growth of the test organism; to identify which extract has been effective in inhibiting the growth of test organism; and to determine the significant difference between the antimicrobial activity of Koro-korosan extracts and the commercially available antifungal and antibacterial drugs.
The antibacterial activity of Koro- korosan (Chloris barbata (Linn.)) leaf and root extract was determined using Agar Cup Plate method. Result showed that only the leaves extracted from hexane shows significant result against Serratia marcescens measuring 6.5mm while the other extracts shows nil or equivalent to zero zone of inhibition. Results revealed that the leaves of Koro- korosan was more effective than roots in bacterial testing.
The determination of antifungal testing was carried out using Poison Plate Method. The degree of mycelial growth was measured to determine the antifungal activity of the extracts, the smaller the growth compared to the control indicates the effectiveness of the assay. Results revealed that ethanolic and aqueous root extracts showed significant results against Fusarium oxysporum measuring 16.5, 12.5, 16mm in three trials of ethanolic root extract and 18, 16,17.75 mm in three trials of aqueous root extract respectively. The two extracts showed greater activity than the positive control (Fluconazole). Hexane leaves and hexane root extracts showed significant results compared to the negative control. While ethanolic leaf and aqueous root extract did not show any antifungal activity. Results of the assay against Aspergillus niger shows significant result compared to negative control but significantly different from the positive control. This indicates that the extract shows lesser antifungal activity against A. niger compared to F. oxysporum. Hexane leaf extract showed significant results for antibacterial testing against S. marcescens.
On the other hand, antifungal testing showed that ethanolic and aqueous root extracts are significant against F. oxysporum while other extracts showed lesser antifungal activity. Therefore Koro- korosan (Chloris barbata) leaf and root extract can provide good antimicrobial agent against F. oxysporum and S. marcescens.
Submitted copy to the University Library. 02/14/2017 T-6142