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Isolation, characterization and antibiotic-sensitivity testing of enterohemolysin-producing Escherichi coli 0157 from fecal samples of apparently heal by Norman V. Solis

By:

Solis, Norman V [author]

Contributor(s):

Rundina- dela Cruz, Cynthia N [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2007. Cavite State University- Main Campus,Description: xvi, 83 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

636.408 So4 2007

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biomedical Sciences (VETMET)

Abstract: SOLIS, Norman V. April 2007. Cavite State University, Indang, Cavite, Isolation, Characterization and Antibiotic-Sensitivity Testing of Enterohemolysin producing Escherichia coli 0157 from Fecal Samples of Apparently Healthy Grower Pigs in Swine Farms in Lemery and Sta. Teresita, Batangas. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser: Ma. Cynthia N. Rundina - dela Cruz, DVM, MS. The study was done to isolate and characterize enterohemolysin - producing Escherichia coli 0157 in rectal swabs of 200 healthy grower pigs in selected farms in Batangas, determine the prevalence of enterohemolysin-producing E. coli 0157 and perform antibiotic sensitivity test on the isolates using selected antimicrobials. One hundred-seventy eight colonies were isolated from Sorbitol MacConkey Agar (Acumedia@) supplemented with cefixime and tellurite (Dynal@). Morphological characterization revealed that where 171 of the 178 isolates were Gram negative, rod- shaped organisms. The isolates were further characterized by biochemical tests and 65 of the 171 possessed the following E. coli reactions: Oxidase negative, Acid slant/ Acid butt with gas production on Triple Sugar Iron, no hydrogen sulfide production, indole positive and non-motile on Sulfide Indole Motility Medium, methyl red positive and Voges-Proskauer test negative, citrate utilization negative, lysine decarboxylase positive, urease negative, gelatin utilization negative. However, 19 of the 65 isolates did not oxidise nor ferment sorbitol. Serologically, only 8 isolates were identified as E. coli 0157:non-H7 wherein, enterohemolysin was detected in all except 2 isolates using washed sheep red blood cells. All isolates were found to be susceptible to fosfomycin, ciprofloxacin, norfloxacin, doxycycline, colistin, and amoxicillin, but resistant to streptomycin and trimethoprim- sulphamethoxazole. The presence of enterohemolysin-producing E Coli 0157 suggests a potential role in causing disease in swine and humans.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	636.408 So4 2007 (Browse shelf(Opens below))		Link to resource	Room use only	T-3471		00007307

Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

SOLIS, Norman V. April 2007. Cavite State University, Indang, Cavite, Isolation, Characterization and Antibiotic-Sensitivity Testing of Enterohemolysin producing Escherichia coli 0157 from Fecal Samples of Apparently Healthy Grower Pigs in Swine Farms in Lemery and Sta. Teresita, Batangas. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser: Ma. Cynthia N. Rundina - dela Cruz, DVM, MS.
The study was done to isolate and characterize enterohemolysin - producing Escherichia coli 0157 in rectal swabs of 200 healthy grower pigs in selected farms in
Batangas, determine the prevalence of enterohemolysin-producing E. coli 0157 and
perform antibiotic sensitivity test on the isolates using selected antimicrobials. One hundred-seventy eight colonies were isolated from Sorbitol MacConkey Agar (Acumedia@) supplemented with cefixime and tellurite (Dynal@). Morphological characterization revealed that where 171 of the 178 isolates were Gram negative, rod- shaped organisms. The isolates were further characterized by biochemical tests and 65 of the 171 possessed the following E. coli reactions: Oxidase negative, Acid slant/ Acid butt with gas production on Triple Sugar Iron, no hydrogen sulfide production, indole positive and non-motile on Sulfide Indole Motility Medium, methyl red positive and Voges-Proskauer test negative, citrate utilization negative, lysine decarboxylase positive, urease negative, gelatin utilization negative. However, 19 of the 65 isolates did not oxidise nor ferment sorbitol. Serologically, only 8 isolates were identified as E. coli 0157:non-H7 wherein, enterohemolysin was detected in all except 2 isolates using washed sheep red blood cells. All isolates were found to be susceptible to fosfomycin, ciprofloxacin, norfloxacin, doxycycline, colistin, and amoxicillin, but resistant to streptomycin and trimethoprim- sulphamethoxazole. The presence of enterohemolysin-producing E Coli 0157 suggests a potential role in causing disease in swine and humans.

Submitted to the University Library 04-10-2007 T-3471