Antibiotoc sensitivity profile of hemolysin producing Escherichia coli 0157 : H7 in native calves from selected farms in Cavite / by Joycedell I. Racosa.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2008. Cavite State University- Main Campus,Description: xiv, 60 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.2  R11 2008
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: RACASA JOYCEDELL I., Cavite State University, Indang, Cavite, April 2008. Antibiotic Sensitivity Profile of Hemolysin-Producing Escherichia coli 0157:H7.Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser: Ma. Cynthia N. Rundina - dela Cruz, DVM, MS. The study was conducted to determine the antibiotic profile of Escherichia coli 0157:H7 in rectal faecal samples of native calves. Sixty five colonies were isolated from Sorbitol MacConkey Agar (Acumedia®) supplemented with cefixime and tellurite (Dynal®). In morphological characterization it showed that 47 isolates were gram negative, rod-shaped organisms out of 65 isolates. The isolates were characterised biochemically and were found to have the following E. coli reactions: Oxidase negative (-), Acid slant/ Acid butt with gas production on Triple Sugar Iron, absence of hydrogen sulphide production, indole positive and are motile or non- motile on Sulfide Indole Motility Medium, Methyl-red positive (+) and Voges-Proskauer test negative (-), citrate utilisation negative (-), lysine decarboxylase positive (+), urease negative (-) and gelatinase negative. Furthermore, the isolates were subjected to carbohydrate utilization test and 22 isolates oxidized and fermented dextrose (Difco®), while 20 isolates fermented and oxidized lactose (Pronadisa®) and maltose (Sigma®). Only 1 isolate did not oxidize or ferment the sorbitol sugar and were considered as presumptive Escherichia coli O157:H7.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.2 R11 2008 (Browse shelf(Opens below)) Link to resource Room use only T-3735 00007495

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

RACASA JOYCEDELL I., Cavite State University, Indang, Cavite, April 2008. Antibiotic Sensitivity Profile of Hemolysin-Producing Escherichia coli 0157:H7.Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser: Ma. Cynthia
N. Rundina - dela Cruz, DVM, MS.

The study was conducted to determine the antibiotic profile of Escherichia coli 0157:H7 in rectal faecal samples of native calves. Sixty five colonies were isolated from Sorbitol MacConkey Agar (Acumedia®) supplemented with cefixime and tellurite (Dynal®). In morphological characterization it showed that 47 isolates were gram negative, rod-shaped organisms out of 65 isolates. The isolates were characterised biochemically and were found to have the following E. coli reactions: Oxidase negative (-), Acid slant/ Acid butt with gas production on Triple Sugar Iron, absence of hydrogen sulphide production, indole positive and are motile or non- motile on Sulfide Indole Motility Medium, Methyl-red positive (+) and Voges-Proskauer test negative (-), citrate utilisation negative (-), lysine decarboxylase positive (+), urease negative (-) and gelatinase negative.

Furthermore, the isolates were subjected to carbohydrate utilization test and 22 isolates oxidized and fermented dextrose (Difco®), while 20 isolates fermented and oxidized lactose (Pronadisa®) and maltose (Sigma®).

Only 1 isolate did not oxidize or ferment the sorbitol sugar and were considered as presumptive Escherichia coli O157:H7.

Submitted to the University Library 04-03-2008 T-3735

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