Normal view MARC view ISBD view

Heavy metal tolerance and antibiotics resistance patterns of bacteria from selected rivers of Cavite / by Jamaica V. Quiñones.

By:

Quiñones, Jamaica V [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xvii, 176 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

551.4 Q4 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

Graduate Studies Open Learning College (GSOLC)

Abstract: QUINONES, JAMAICA V. “Heavy Metal Tolerance and Antibiotic Resistance Patterns of Bacteria from Selected Rivers of Cavite”. Graduate Thesis, Master of Science in Biology, Graduate School and Open Learning College, Cavite State University, Indang, Cavite. May 2017. Adviser: Dr. Yolanda A. Ilagan. This study aimed to determine the physico-chemical characteristics of water from selected rivers of Cavite, identify the bacteria present, ascertain the bacterial genus most tolerant to metals and most resistant to antibiotics, determine the percentage of metal and antibiotic resistant bacterial isolates, analyze their metal tolerance and antibiotic resistance patterns, and correlate their metal tolerance with their antibiotic resistance. Water samples were taken from Ikloy, Lit-litan, Kaong, Maragondon, Pulunan, Puting Tubig, Ylang-Ylang, Cafias, and Dasmarifias Rivers. pH, dissolved oxygen (DO), biological oxygen demand (BOD), and total dissolved solids (TDS) of these rivers were measured. DO, TDS, and pH of the rivers were within the DENR standard except for the pH of Pulunan River. Only Kaong River exceeded the DENR standard for BOD. In the four media used in this study, growth on TYGA had an average count of 1.18 x 10’ and samples from Gen. Trias (Ylang-Ylang River) and Trece Martires City A (Pulunan River) had the highest (8.2 x 10°) and lowest (6.1 x 10‘) counts, respectively. Meanwhile, growth on CA had an average count of 3.23 x 10° cfu/ml with the highest count of 2.0 x 10° in sample from Gen. Trias (Ylang-Ylang) and lowest count of 3.0 x 107 in sample from Ternate (Maragondon River). Bacterial count in TGYA had a positive significant correlation with biological oxygen demand (BOD) and a negative significant correlation with dissolved oxygen (DO). On the other hand, bacterial count in MCA had a positive significant correlation with total dissolved solids (TDS). A total of 190 bacteria were isolated from these rivers and identified to be under the genera Bacillus, Corynebacterium, Pseudomonas, Aeromonas, Serratia, Klebsiella, Lnterobacter, Yersinia, Shigella, Proteus, Providencia, Morganella, Citrobacter, E. coli Micrococcus, and Staphylococcus based on morphological and_ physiological characteristics. One, however, remained unidentified. These bacterial isolates were tested for tolerance against six heavy metals (zinc, lead, copper, mercury, manganese, and iron) at varying concentrations (100, 150, 200, 250, 400, 800, and 1600 ppm) and for resistance against 100 ppm of ten antibiotics (ampicillin, erythromycin, streptomycin, tetracycline, kanamycin, ofloxacin, clindamycin, oxytetracycline, levofloxacin, and ceftriaxone). Among the six heavy metals tested, iron and manganese were the most tolerated. Serratia was observed to be the most tolerant genus to lead, copper, zinc, manganese and iron while the least tolerant to mercury. Four metal tolerance patterns were exhibited by the bacterial isolates with penta-metal tolerance as the main pattern. Bacterial isolates also exhibited antibiotic resistance with resistance to oxytetracycline and tetracycline as the most common. Serratia was observed to be the most resistant genus to the antibiotics erythromycin, clindamycin, and ceftriaxone. Aeromonas was the most resistant genus to kanamycin. Meanwhile, both Serratia and Aeromonus were the most resistant genera against the antibiotics ampicillin and tetracycline. Staphylococcus was the most resistant to both fluoroquinolone antibiotics: ofloxacin and levofloxacin and pseudomonas to streptomycin. In oxytetracycline, almost all the genera with at least ten isolates were resistant to this antibiotic except for Pseudomonas. One hundred eighty four of the 190 isolates were resistant to three or more antibiotics. Eight antibiotic resistance patterns were observed with hepta-antibiotic resistance as the main pattern. Tolerance to copper, mercury and zinc had a highly significant relationship (P<0.05) with resistance to the antibiotic, tetracycline but otherwise to ofloxacin, kanamycin, and levofloxacin.

Average rating: 0.0 (0 votes)

Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	551.4 Q4 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6720		00010957

Thesis (Master of Science in Biology) Cavite State University

Includes bibliographical references.

Graduate Studies Open Learning College (GSOLC)

QUINONES, JAMAICA V. “Heavy Metal Tolerance and Antibiotic Resistance Patterns of Bacteria from Selected Rivers of Cavite”. Graduate Thesis, Master of Science in Biology, Graduate School and Open Learning College, Cavite State University, Indang, Cavite. May 2017. Adviser: Dr. Yolanda A. Ilagan.

This study aimed to determine the physico-chemical characteristics of water from selected rivers of Cavite, identify the bacteria present, ascertain the bacterial genus most tolerant to metals and most resistant to antibiotics, determine the percentage of metal and antibiotic resistant bacterial isolates, analyze their metal tolerance and antibiotic resistance patterns, and correlate their metal tolerance with their antibiotic resistance.

Water samples were taken from Ikloy, Lit-litan, Kaong, Maragondon, Pulunan, Puting Tubig, Ylang-Ylang, Cafias, and Dasmarifias Rivers. pH, dissolved oxygen (DO), biological oxygen demand (BOD), and total dissolved solids (TDS) of these rivers were measured. DO, TDS, and pH of the rivers were within the DENR standard except for the pH of Pulunan River. Only Kaong River exceeded the DENR standard for BOD.

In the four media used in this study, growth on TYGA had an average count of 1.18 x 10’ and samples from Gen. Trias (Ylang-Ylang River) and Trece Martires City A (Pulunan River) had the highest (8.2 x 10°) and lowest (6.1 x 10‘) counts, respectively. Meanwhile, growth on CA had an average count of 3.23 x 10° cfu/ml with the highest count of 2.0 x 10° in sample from Gen. Trias (Ylang-Ylang) and lowest count of 3.0 x 107 in sample from Ternate (Maragondon River).

Bacterial count in TGYA had a positive significant correlation with biological oxygen demand (BOD) and a negative significant correlation with dissolved oxygen (DO). On the other hand, bacterial count in MCA had a positive significant correlation
with total dissolved solids (TDS).

A total of 190 bacteria were isolated from these rivers and identified to be under the genera Bacillus, Corynebacterium, Pseudomonas, Aeromonas, Serratia, Klebsiella, Lnterobacter, Yersinia, Shigella, Proteus, Providencia, Morganella, Citrobacter, E. coli Micrococcus, and Staphylococcus based on morphological and_ physiological
characteristics. One, however, remained unidentified.

These bacterial isolates were tested for tolerance against six heavy metals (zinc, lead, copper, mercury, manganese, and iron) at varying concentrations (100, 150, 200, 250, 400, 800, and 1600 ppm) and for resistance against 100 ppm of ten antibiotics (ampicillin, erythromycin, streptomycin, tetracycline, kanamycin, ofloxacin,
clindamycin, oxytetracycline, levofloxacin, and ceftriaxone).

Among the six heavy metals tested, iron and manganese were the most tolerated. Serratia was observed to be the most tolerant genus to lead, copper, zinc, manganese and iron while the least tolerant to mercury. Four metal tolerance patterns were exhibited by the bacterial isolates with penta-metal tolerance as the main pattern.

Bacterial isolates also exhibited antibiotic resistance with resistance to oxytetracycline and tetracycline as the most common. Serratia was observed to be the most resistant genus to the antibiotics erythromycin, clindamycin, and ceftriaxone. Aeromonas was the most resistant genus to kanamycin. Meanwhile, both Serratia and Aeromonus were the most resistant genera against the antibiotics ampicillin and tetracycline. Staphylococcus was the most resistant to both fluoroquinolone antibiotics: ofloxacin and levofloxacin and pseudomonas to streptomycin. In oxytetracycline, almost all the genera with at least ten isolates were resistant to this antibiotic except for Pseudomonas. One hundred eighty four of the 190 isolates were resistant to three or more antibiotics. Eight antibiotic resistance patterns were observed with hepta-antibiotic resistance as the main pattern.

Tolerance to copper, mercury and zinc had a highly significant relationship (P<0.05) with resistance to the antibiotic, tetracycline but otherwise to ofloxacin, kanamycin, and levofloxacin.

Submitted copy to the University Library. 07/22/2017 T-6720