Prevalence, phenotypic variabilities and anti-biograms of methicillin-resistant (staphylococcus aureus (MRSA) among nursing students of Cavite State University / by Anthony D. Galisanao

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2008. Cavite State University- Main Campus,Description: xiii, 74 pages : 28 cm. illustrationsContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 616.9  G13 2008
Online resources: Production credits:
  • College of Arts and Science (CAS)
Abstract: STO. DOMINGO, MA. RODEZ SIWA. PREVALENCE, PHENOTYPIC VARIABILITIES AND ANTIBIOGRAMS OF METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) AMONG HOSPITAL PATIENTS OF REPRODUCTIVE AGE. Undergraduate Thesis, Bachelor of Science in Biology. Cavite State University, Indang, Cavite. April 2008. Adviser: Dr. Yolanda A. Hagan. The study was conducted to assess the prevalence rate, phenotypic variabilities and antibiograms of MRSA among hospital patients of reproductive age in the province of Cavite. One hundred and sixty-five respondents were interviewed and provided with nasal swabs. Out of the subjects who participated in the study, 49 (41.82%) were positive for S. aureus. The prevalence rate of MRSA was 21.82%. All S. aureus isolates were circular, convex and with entire margin. They appearedin different colors: whitish yellow (72.22%), greenish yellow (5.56%) and yellow (22.22%); and hemolysis: non-hemolytic or gamma hemolysis pattern were demonstrated by the 38.89% of MRSA isolates, while thirteen (36.11%) and nine (25%) isolates were distinguished as f3-hemolytic and alpha-hemolytic, respectively, when grown on Blood Agar Plates. The MRSA isolated from hospital patients of reproductive age were highly susceptible (25% - 94.44%) to gentamicin, kanamycin, streptomycin, ciprofloxacin, vancomycin, clindamycin, lincomycin, erythromycin, nalidixic acid, norfloxacin, rifampicin, chloramphenicol and tetracycline compared to penicillin, amoxicillin-clavulanic acid and ampicillin with 22.22% - 27.78%. MRSA isolates exhibited higher rate of intermediate resistance and resistance to [3-lactams (27.78% - 75%) than to non-[3- lactams (0% - 58.33%). Thirty-two isolates exhibited multidrug resistance pattern. The patterns were classified as mono-resistance (25%), bi-resistance (34.38%), tri-resistance (18.75%), tetra-resistance (15.63%), penta-resistance (3.13%), nona-resistance (3.13%), and hendeca-resistance (3.13%). The Minimum Inhibitory Concentration (MIC) of D-lactam and non-fi-lactam antibiotics to MRSA isolates ranged from 16 to164 pg/mL. The surveyed demographic characteristics such as classification, gender, age, civil status, address, residential area, occupation, estimated family monthly income, number of siblings and number of children were not significantly correlated with MRS acquisition. The duration of hospitalization, on the other hand, was significantly correlated with acquisition of MRSA infection.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 616.9 G13 2008 (Browse shelf(Opens below)) Link to resource Room use only T-3816 00007458

Thesis (BS Biology - - Microbiology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

STO. DOMINGO, MA. RODEZ SIWA. PREVALENCE, PHENOTYPIC VARIABILITIES AND ANTIBIOGRAMS OF METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) AMONG HOSPITAL PATIENTS OF REPRODUCTIVE AGE. Undergraduate Thesis, Bachelor of Science in Biology. Cavite State University, Indang, Cavite. April 2008. Adviser: Dr. Yolanda A. Hagan.
The study was conducted to assess the prevalence rate, phenotypic variabilities and antibiograms of MRSA among hospital patients of reproductive age in the province of Cavite. One hundred and sixty-five respondents were interviewed and provided with nasal swabs. Out of the subjects who participated in the study, 49 (41.82%) were positive for S. aureus. The prevalence rate of MRSA was 21.82%. All S. aureus isolates were circular, convex and with entire margin. They appearedin different colors: whitish yellow (72.22%), greenish yellow (5.56%) and yellow (22.22%); and hemolysis: non-hemolytic or gamma hemolysis pattern were demonstrated by the 38.89% of MRSA isolates, while thirteen (36.11%) and nine (25%) isolates were distinguished as f3-hemolytic and alpha-hemolytic, respectively, when grown on Blood Agar Plates. The MRSA isolated from hospital patients of reproductive age were highly susceptible (25% - 94.44%) to gentamicin, kanamycin, streptomycin, ciprofloxacin, vancomycin, clindamycin, lincomycin, erythromycin, nalidixic acid, norfloxacin, rifampicin, chloramphenicol and tetracycline compared to penicillin, amoxicillin-clavulanic acid and ampicillin with 22.22% - 27.78%. MRSA isolates exhibited higher rate of intermediate resistance and resistance to [3-lactams (27.78% - 75%) than to non-[3- lactams (0% - 58.33%).
Thirty-two isolates exhibited multidrug resistance pattern. The patterns were classified as mono-resistance (25%), bi-resistance (34.38%), tri-resistance (18.75%), tetra-resistance (15.63%), penta-resistance (3.13%), nona-resistance (3.13%), and hendeca-resistance (3.13%). The Minimum Inhibitory Concentration (MIC) of D-lactam and non-fi-lactam antibiotics to MRSA isolates ranged from 16 to164 pg/mL. The surveyed demographic characteristics such as classification, gender, age, civil status, address, residential area, occupation, estimated family monthly income, number of siblings and number of children were not significantly correlated with MRS acquisition. The duration of hospitalization, on the other hand, was significantly correlated with acquisition of MRSA infection.



Submitted to the University Library 06/10/2008 T-3816

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