MARC details
000 -LEADER |
fixed length control field |
03692nam a2200313 a 4500 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
OSt |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20230422110903.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
070508s19uu xx 00 eng d |
040 ## - CATALOGING SOURCE |
Transcribing agency |
CvSU Main Campus Library |
041 0# - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
Classification number |
636.293 |
Item number |
P92 2005 |
100 #0 - MAIN ENTRY--PERSONAL NAME |
Personal name |
Presa, Judy S. |
9 (RLIN) |
10588 |
Relator term |
author |
245 10 - TITLE STATEMENT |
Title |
Detection of shiga (vero) toxin producing E-coli 0157 in carabaos at the Philippine Carabao Center / |
Statement of responsibility, etc. |
by Judy S. Presa. |
260 0# - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
Indang, Cavite : |
Date of publication, distribution, etc. |
2005. |
Name of publisher, distributor, etc. |
Cavite State University- Main Campus, |
300 ## - PHYSICAL DESCRIPTION |
Extent |
xv, 49 pages : |
Other physical details |
illustrations ; |
Dimensions |
28 cm. |
336 ## - CONTENT TYPE |
Source |
rdacontent |
Content type term |
text |
337 ## - MEDIA TYPE |
Source |
rdamedia |
Media type term |
unmediated |
338 ## - CARRIER TYPE |
Source |
rdacarrier |
Carrier type term |
volume |
500 ## - GENERAL NOTE |
General note |
Thesis (Doctor of Veterinary Medicine) Cavite State University |
504 ## - BIBLIOGRAPHY, ETC. NOTE |
Bibliography, etc. note |
Includes bibliographical references. |
508 ## - CREATION/PRODUCTION CREDITS NOTE |
College and Department |
College of Veterinary Medicine and Biomedical Sciences (VETMET) |
520 3# - SUMMARY, ETC. |
Summary, etc. |
ABSTRACT <br/>PRESA JUDY SUSANA. Cavite State University, lndang, Cavite. April 2005. Detection of Shiga (Vero) Toxin-Producing Escherichia coli 0157 In Carabaos (bubalus bubalis) at the Philippine Carabao Center. Undergraduate Thesis. Doctor of Veterinary Medicine. Adviser: Ma. Cynthia N. Rundina, DVM MS. <br/><br/>This study was done to detect Shiga (Vero) toxin-producing Escherichia coli 0157 from the rectal fecal samples of 66 carabaos (Bubalus bubalis). <br/><br/>The rectal fecal samples were subjected to direct culture onto Cefixime Tellurite-Sorbitol MacConkey Agar (CT-SMAC) and were characterized culturally on the basis of its inability to Ferment sorbitol. A total of 89 colorless colonies were isolated and were considered as presumptive Enterohemorrhagic Escherichia coli (EHEC) and were Further characterized morphologically.<br/><br/>Microscopically. all isolates Were gram-negative and rod-shaped organisms. These isolates were then tested further biochemically and 36 (40.4%) produced the following typical reactions: Oxidase negative (-), Acid slant and Acid butt with gas production (A/A gas) in Triple Sugar Iron TSI) agar, no hydrogen sulfide production and motile in Sulfide Indole Motility (SIM) medium, urease negative (-), citrate utilization negative (-)Voges Proskauer test negative (-), methyl red positive ( ). indole positive) and Ivsinc decarboxylase positive (+). Moreover, these isolates ferment the ramvanu sugar: glucose. lactose and maltose. <br/><br/>Only 2 (5.6%) 01 the presumptive FM' isolates did not oxidize or Ferment sorbitol in the sorbitol utilization test. These isolates were considered as presumptive Escherichia colil 0157 and were tested further for agglutination with the 0157 antiserum. <br/><br/>All of the isolates did not agglutinate the O157 antiserum and further testing using the Polymerase Chain Reaction (PCR) with the BIOTECH primers Code 12 and 13 showed that none of the isolates amplified the primers. Moreover, PCR results showed that none ol' the isolates amplified the // primer. These results showed that none of the isolates were O157 and none of these isolates possessed the Shiva (Vero) toxin. <br/><br/>It is apparent based on the results of this study that Dia' strains other than E. coli O157 may be more evident in buffalo population and that further research these strains are warranted as they may also possess the Shiga (Vero) toxin.<br/> |
541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE |
Method of acquisition |
Submitted to the University Library |
Date of acquisition |
05/10/2007 |
Accession number |
T-2991 |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Carabaos |
9 (RLIN) |
9680 |
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) |
9 (RLIN) |
66 |
Course or Program |
Doctor of Veterinary Medicine |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Rundina- dela Cruz, Cynthia N. |
9 (RLIN) |
29399 |
Relator term |
adviser |
856 ## - ELECTRONIC LOCATION AND ACCESS |
Electronic File |
<a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=6e21880a1d71fdc25533be5d1c5374e2">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=6e21880a1d71fdc25533be5d1c5374e2</a> |
Link text |
Click here to view abstract and table of contents |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Theses / Manuscripts |