Detection of shiga (vero) toxin producing E-coli 0157 in carabaos at the Philippine Carabao Center / (Record no. 6601)

MARC details
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fixed length control field 03692nam a2200313 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230422110903.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 070508s19uu xx 00 eng d
040 ## - CATALOGING SOURCE
Transcribing agency CvSU Main Campus Library
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 636.293
Item number P92 2005
100 #0 - MAIN ENTRY--PERSONAL NAME
Personal name Presa, Judy S.
9 (RLIN) 10588
Relator term author
245 10 - TITLE STATEMENT
Title Detection of shiga (vero) toxin producing E-coli 0157 in carabaos at the Philippine Carabao Center /
Statement of responsibility, etc. by Judy S. Presa.
260 0# - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Indang, Cavite :
Date of publication, distribution, etc. 2005.
Name of publisher, distributor, etc. Cavite State University- Main Campus,
300 ## - PHYSICAL DESCRIPTION
Extent xv, 49 pages :
Other physical details illustrations ;
Dimensions 28 cm.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
500 ## - GENERAL NOTE
General note Thesis (Doctor of Veterinary Medicine) Cavite State University
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references.
508 ## - CREATION/PRODUCTION CREDITS NOTE
College and Department College of Veterinary Medicine and Biomedical Sciences (VETMET)
520 3# - SUMMARY, ETC.
Summary, etc. ABSTRACT <br/>PRESA JUDY SUSANA. Cavite State University, lndang, Cavite. April 2005. Detection of Shiga (Vero) Toxin-Producing Escherichia coli 0157 In Carabaos (bubalus bubalis) at the Philippine Carabao Center. Undergraduate Thesis. Doctor of Veterinary Medicine. Adviser: Ma. Cynthia N. Rundina, DVM MS. <br/><br/>This study was done to detect Shiga (Vero) toxin-producing Escherichia coli 0157 from the rectal fecal samples of 66 carabaos (Bubalus bubalis). <br/><br/>The rectal fecal samples were subjected to direct culture onto Cefixime Tellurite-Sorbitol MacConkey Agar (CT-SMAC) and were characterized culturally on the basis of its inability to Ferment sorbitol. A total of 89 colorless colonies were isolated and were considered as presumptive Enterohemorrhagic Escherichia coli (EHEC) and were Further characterized morphologically.<br/><br/>Microscopically. all isolates Were gram-negative and rod-shaped organisms. These isolates were then tested further biochemically and 36 (40.4%) produced the following typical reactions: Oxidase negative (-), Acid slant and Acid butt with gas production (A/A gas) in Triple Sugar Iron TSI) agar, no hydrogen sulfide production and motile in Sulfide Indole Motility (SIM) medium, urease negative (-), citrate utilization negative (-)Voges Proskauer test negative (-), methyl red positive ( ). indole positive) and Ivsinc decarboxylase positive (+). Moreover, these isolates ferment the ramvanu sugar: glucose. lactose and maltose. <br/><br/>Only 2 (5.6%) 01 the presumptive FM' isolates did not oxidize or Ferment sorbitol in the sorbitol utilization test. These isolates were considered as presumptive Escherichia colil 0157 and were tested further for agglutination with the 0157 antiserum. <br/><br/>All of the isolates did not agglutinate the O157 antiserum and further testing using the Polymerase Chain Reaction (PCR) with the BIOTECH primers Code 12 and 13 showed that none of the isolates amplified the primers. Moreover, PCR results showed that none ol' the isolates amplified the // primer. These results showed that none of the isolates were O157 and none of these isolates possessed the Shiva (Vero) toxin. <br/><br/>It is apparent based on the results of this study that Dia' strains other than E. coli O157 may be more evident in buffalo population and that further research these strains are warranted as they may also possess the Shiga (Vero) toxin.<br/>
541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE
Method of acquisition Submitted to the University Library
Date of acquisition 05/10/2007
Accession number T-2991
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Carabaos
9 (RLIN) 9680
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
9 (RLIN) 66
Course or Program Doctor of Veterinary Medicine
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Rundina- dela Cruz, Cynthia N.
9 (RLIN) 29399
Relator term adviser
856 ## - ELECTRONIC LOCATION AND ACCESS
Electronic File <a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=6e21880a1d71fdc25533be5d1c5374e2">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=6e21880a1d71fdc25533be5d1c5374e2</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Theses / Manuscripts
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Home library Current library Shelving location Date acquired Source of acquisition Coded location qualifier Inventory number Total Renewals Full call number Barcode Date last seen Uniform Resource Identifier Price effective from Koha item type Public note
    Dewey Decimal Classification   Room use only Non-fiction Ladislao N. Diwa Memorial Library Ladislao N. Diwa Memorial Library Theses Section 05/10/2007 Submitted copy to the University Library THESIS/SP 600 2005 636.293 P92 2005 00006600 08/04/2020 http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=6e21880a1d71fdc25533be5d1c5374e2 08/04/2020 Theses / Manuscripts T-2991
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