Detection of shiga (vero) toxin producing E-coli 0157 in carabaos at the Philippine Carabao Center / by Judy S. Presa.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2005. Cavite State University- Main Campus,Description: xv, 49 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.293  P92 2005
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: ABSTRACT PRESA JUDY SUSANA. Cavite State University, lndang, Cavite. April 2005. Detection of Shiga (Vero) Toxin-Producing Escherichia coli 0157 In Carabaos (bubalus bubalis) at the Philippine Carabao Center. Undergraduate Thesis. Doctor of Veterinary Medicine. Adviser: Ma. Cynthia N. Rundina, DVM MS. This study was done to detect Shiga (Vero) toxin-producing Escherichia coli 0157 from the rectal fecal samples of 66 carabaos (Bubalus bubalis). The rectal fecal samples were subjected to direct culture onto Cefixime Tellurite-Sorbitol MacConkey Agar (CT-SMAC) and were characterized culturally on the basis of its inability to Ferment sorbitol. A total of 89 colorless colonies were isolated and were considered as presumptive Enterohemorrhagic Escherichia coli (EHEC) and were Further characterized morphologically. Microscopically. all isolates Were gram-negative and rod-shaped organisms. These isolates were then tested further biochemically and 36 (40.4%) produced the following typical reactions: Oxidase negative (-), Acid slant and Acid butt with gas production (A/A gas) in Triple Sugar Iron TSI) agar, no hydrogen sulfide production and motile in Sulfide Indole Motility (SIM) medium, urease negative (-), citrate utilization negative (-)Voges Proskauer test negative (-), methyl red positive ( ). indole positive) and Ivsinc decarboxylase positive (+). Moreover, these isolates ferment the ramvanu sugar: glucose. lactose and maltose. Only 2 (5.6%) 01 the presumptive FM' isolates did not oxidize or Ferment sorbitol in the sorbitol utilization test. These isolates were considered as presumptive Escherichia colil 0157 and were tested further for agglutination with the 0157 antiserum. All of the isolates did not agglutinate the O157 antiserum and further testing using the Polymerase Chain Reaction (PCR) with the BIOTECH primers Code 12 and 13 showed that none of the isolates amplified the primers. Moreover, PCR results showed that none ol' the isolates amplified the // primer. These results showed that none of the isolates were O157 and none of these isolates possessed the Shiva (Vero) toxin. It is apparent based on the results of this study that Dia' strains other than E. coli O157 may be more evident in buffalo population and that further research these strains are warranted as they may also possess the Shiga (Vero) toxin.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.293 P92 2005 (Browse shelf(Opens below)) Link to resource Room use only T-2991 00006600

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

ABSTRACT
PRESA JUDY SUSANA. Cavite State University, lndang, Cavite. April 2005. Detection of Shiga (Vero) Toxin-Producing Escherichia coli 0157 In Carabaos (bubalus bubalis) at the Philippine Carabao Center. Undergraduate Thesis. Doctor of Veterinary Medicine. Adviser: Ma. Cynthia N. Rundina, DVM MS.

This study was done to detect Shiga (Vero) toxin-producing Escherichia coli 0157 from the rectal fecal samples of 66 carabaos (Bubalus bubalis).

The rectal fecal samples were subjected to direct culture onto Cefixime Tellurite-Sorbitol MacConkey Agar (CT-SMAC) and were characterized culturally on the basis of its inability to Ferment sorbitol. A total of 89 colorless colonies were isolated and were considered as presumptive Enterohemorrhagic Escherichia coli (EHEC) and were Further characterized morphologically.

Microscopically. all isolates Were gram-negative and rod-shaped organisms. These isolates were then tested further biochemically and 36 (40.4%) produced the following typical reactions: Oxidase negative (-), Acid slant and Acid butt with gas production (A/A gas) in Triple Sugar Iron TSI) agar, no hydrogen sulfide production and motile in Sulfide Indole Motility (SIM) medium, urease negative (-), citrate utilization negative (-)Voges Proskauer test negative (-), methyl red positive ( ). indole positive) and Ivsinc decarboxylase positive (+). Moreover, these isolates ferment the ramvanu sugar: glucose. lactose and maltose.

Only 2 (5.6%) 01 the presumptive FM' isolates did not oxidize or Ferment sorbitol in the sorbitol utilization test. These isolates were considered as presumptive Escherichia colil 0157 and were tested further for agglutination with the 0157 antiserum.

All of the isolates did not agglutinate the O157 antiserum and further testing using the Polymerase Chain Reaction (PCR) with the BIOTECH primers Code 12 and 13 showed that none of the isolates amplified the primers. Moreover, PCR results showed that none ol' the isolates amplified the // primer. These results showed that none of the isolates were O157 and none of these isolates possessed the Shiva (Vero) toxin.

It is apparent based on the results of this study that Dia' strains other than E. coli O157 may be more evident in buffalo population and that further research these strains are warranted as they may also possess the Shiga (Vero) toxin.

Submitted to the University Library 05/10/2007 T-2991

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