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Molecular detection of Anaplasma spp. on ticks collected from cattle and pasture in Cavite using polymerase chain reaction / by Maarten Czar Legaspi Mojares.

By:

Mojares, Maarten Czar Legaspi [author]

Contributor(s):

Mago, Emmanuel R [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite: Cavite State University - Main Campus 2017.Description: xii, 58 pages illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

630 M81 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biomedical Sciences (VETMED)

Abstract: MOJARES, MAARTEN CZAR L., PCR Detection of Mt-rrs Gene and Anaplasma spp. among Ticks Collected from Cattle and Pasture in Cavite. Undergraduate Thesis/Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite,2017. Adviser: Dr Emmanuel R. Mago This study was conducted as a preliminary survey of the presence of tick borne pathogen Anaplasma spp. in different cattle farms in Cavite, Philippines using PCR. A total of 293 adult ticks and 1 larva samples were collected in which 63 (21.42%) came from lowland and 231 (78.57%) from central hilly areas in Cavite. Ticks were identified and classified according to species, sex, development stage and topography. Eighty nine samples were tested using blackPrep Tick Kit and 205 samples using Boiling method for Mt-rrs gene detection. Eighty (89.89% ) samples used in blackPrep Tick Kit were positive for the gene while 85 (41.46°A) samples including 1 larva in Boiling method. All 165 mt-rrs gene positive samples were tested for Anaplasma spp. detection but failed to exhibit distinct band on the 513 by mark indicating that the samples were not Anaplasma spp. BlackPrep Tick DNA/RNA kit provided a much higher detection rate than using Boiling method in detection of Mt-rrs gene. The presence of three genes in Rhipicephalus microplus namely CK187220, CV437619 and TC18492 might have been a contributing factor in failure to detect Anapalsma spp. Use of Serum collected from cattle would greatly help in detecting the presence of Anaplasma spp. in farms in Cavite. Different species of ticks should be used as sample to avoid bias in the result. The use of gradient PCR would increase the specificity of detection thus providing a more reasonable result.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	630 M81 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-7284		00017787

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Previous	630 M31 2022 Awareness, acceptance, congruency and dissemination of the University Vision and Mission, CAFENR goals and objectives of the BS Agricultural program /	630 M41 1996 Sanayan sa kakayahang agrikultura (SAKA) Project Don Severino Agricultural College, Indang, Cavite /	630 M76 2018 Snap beans (Phaseolus vulgaris) barak variety production enterprise grown organically /	630 M81 2017 Molecular detection of Anaplasma spp. on ticks collected from cattle and pasture in Cavite using polymerase chain reaction /	630 N85 1985 Preparation and management in an integrated farming system with emphasis on sloping agricultural land technology (SALT) /	630 P21 2017 Formulation and efficacy testing of fortified vermicompost pellets on the growth of pechay (brassica rapa L.) /	630 R71 1992 Factors associated with the choice of selling practices of farmer-producers in selected municipalities in Quezon Province /	Next

Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMED)

MOJARES, MAARTEN CZAR L., PCR Detection of Mt-rrs Gene and Anaplasma spp. among Ticks Collected from Cattle and Pasture in Cavite. Undergraduate Thesis/Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite,2017. Adviser: Dr Emmanuel R. Mago
This study was conducted as a preliminary survey of the presence of tick borne pathogen Anaplasma spp. in different cattle farms in Cavite, Philippines using PCR. A total of 293 adult ticks and 1 larva samples were collected in which 63 (21.42%) came from lowland and 231 (78.57%) from central hilly areas in Cavite. Ticks were identified and classified according to species, sex, development stage and topography. Eighty nine samples were tested using blackPrep Tick Kit and 205 samples using Boiling method for Mt-rrs gene detection. Eighty (89.89% ) samples used in blackPrep Tick Kit were positive for the gene while 85 (41.46°A) samples including 1 larva in Boiling method. All 165 mt-rrs gene positive samples were tested for Anaplasma spp. detection but failed to exhibit distinct band on the 513 by mark indicating that the samples were not Anaplasma spp. BlackPrep Tick DNA/RNA kit provided a much higher detection rate than using Boiling method in detection of Mt-rrs gene. The presence of three genes in Rhipicephalus microplus namely CK187220, CV437619 and TC18492 might have been a contributing factor in failure to detect Anapalsma spp. Use of Serum collected from cattle would greatly help in detecting the presence of Anaplasma spp. in farms in Cavite. Different species of ticks should be used as sample to avoid bias in the result. The use of gradient PCR would increase the specificity of detection thus providing a more reasonable result.

Submitted to the University Library
08/24/2017 T-7284