Normal view MARC view ISBD view

Biological activities of dichloromethane bark extract of Lumbang (Aleurites moluccana) / Genny Vic A. Dihan.

By:

Dihan, Genny Vic A [author]

Contributor(s):

Saldana, Chester Joshua V [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2012.Description: xv, 70 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

581.634 D56 2012

Online resources:

Click here to view the Abstract and Table of Contents

Production credits:

College of Veterinary Medicine. (DVM), Department of Immunopathology and Microbiology

Abstract: DIHAN, GENNY VIC A., Biological Activities of Dichloromethane Bark Extract of Lumbang (Aleurites moluccana). Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. October 2012, Adviser: Dr. Chester Joshua V. Saldaﬁa, The study was carried out to determine the biological activities of the dichloromethane bark extract of Aleurites molucana. Bioassay, namely antimicrobial assay, carrageenan- induced peritonitis for assessment of anti-inﬂammatory activity, hypoglycemic activity test and brine shrimp lethality were used. Exudates from the peritoneal cavity of mice is characterized by protein-rich ﬂuid accumulation and leukocytes inﬁltration which was evident after 24 hours of carrageenan induced peritonitis. The WBC count signiﬁcantly lower in Aleurites molucana bark extract (12.0 x103/ul) treated group when compared to the number of cells of the negative control (16.3 x 103ml) group but not as signiﬁcant when compared to diclofenac (7.8 x 103/111). Same observation was noted for neutrophil count relative to positive and negative control. In hypoglycemic activity, after oral glucose tolerance test using 20% glucose Aleurites moluccana bark extract signiﬁcantly decreased with percent reduction of 23.37% blood glucose after 30 minutes. Cytotoxic activity was exhibited by Aleurites moluccana bark extract with 100% and 95.19% mortality after exposure to 1000 ug/ml and exposure 100 ug/ml, respectively. The lethal dose 50 (LDso) was established at 10 ug/ml (60.27 %). Moreover, Aleurites moluccana bark extract displayed intermediate anti-bacterial activity against Staphylococcus aureus. .Resistance was observed for Escherichia coli, Pseudomonas aureginosa and Bacillus subtilis when compared to tetracycline (positive control). Weak antifunganl activity against Candida tropicalis and Aspergillus niger was noted relative to positive control (nystatin). Result of the study indicates that the dichloromethane extract of Aleurites molucana bark has signiﬁcant anti-inﬂammatory, hypoglycemic antibacterial and cytotoxic activity. This indicates that there are basis for the popularity of this plant as herbal medicine, This may be attributed to bioactive compounds of the bark extracts of Aleurites molucana such as glycosides, saponins, phenolic compounds and tannins.

Average rating: 0.0 (0 votes)

Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	581.634 D56 2012 (Browse shelf(Opens below))		Link to resource	Room use only	T-4836		00071204

Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Veterinary Medicine. (DVM), Department of Immunopathology and Microbiology

DIHAN, GENNY VIC A., Biological Activities of Dichloromethane Bark Extract of Lumbang (Aleurites moluccana). Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. October 2012, Adviser: Dr. Chester Joshua V. Saldaﬁa,

The study was carried out to determine the biological activities of the dichloromethane bark extract of Aleurites molucana. Bioassay, namely antimicrobial assay, carrageenan- induced peritonitis for assessment of anti-inﬂammatory activity, hypoglycemic activity test and brine shrimp lethality were used.

Exudates from the peritoneal cavity of mice is characterized by protein-rich ﬂuid accumulation and leukocytes inﬁltration which was evident after 24 hours of carrageenan induced peritonitis. The WBC count signiﬁcantly lower in Aleurites molucana bark extract (12.0 x103/ul) treated group when compared to the number of cells of the negative control (16.3 x 103ml) group but not as signiﬁcant when compared to diclofenac (7.8 x 103/111). Same observation was noted for neutrophil count relative to positive and negative control. In hypoglycemic activity, after oral glucose tolerance test using 20% glucose Aleurites moluccana bark extract signiﬁcantly decreased with percent reduction of 23.37% blood glucose after 30 minutes.

Cytotoxic activity was exhibited by Aleurites moluccana bark extract with 100% and 95.19% mortality after exposure to 1000 ug/ml and exposure 100 ug/ml, respectively.
The lethal dose 50 (LDso) was established at 10 ug/ml (60.27 %). Moreover, Aleurites moluccana bark extract displayed intermediate anti-bacterial activity against Staphylococcus aureus. .Resistance was observed for Escherichia coli, Pseudomonas aureginosa and Bacillus subtilis when compared to tetracycline (positive control). Weak antifunganl activity against Candida tropicalis and Aspergillus niger was noted relative to positive control (nystatin).

Result of the study indicates that the dichloromethane extract of Aleurites molucana bark has signiﬁcant anti-inﬂammatory, hypoglycemic antibacterial and cytotoxic activity. This indicates that there are basis for the popularity of this plant as herbal medicine, This may be attributed to bioactive compounds of the bark extracts of Aleurites molucana such as glycosides, saponins, phenolic compounds and tannins.

Submitted to the University Library 12/12/2012 T-4836