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Antibiotic sensitivity profile of salmonella spp. isolates in the gallbladder of chickens (Gallus gallus domesticus. Linn) from a dressing plant in Trece Martirez City, Cavite by Ellen Grace M. Batiles

By:

Batiles, Ellen Grace M [author]

Contributor(s):

Saldana, Chester Joshua V [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2009.Description: xiv, 83 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

636.513 B32e 2009

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biomedical Sciences (VETMET)

Abstract: BATILES, ELLEN GRACE M., Cavite State University, Indang, Cavite, April 2009. Antibiotic Sensitivitv Profile of Salmonella spp. isolates in the gallbladder of chicken (Gallus doinesticus, Lim) from a Dressing Plant in Trece Martirez City, Cavite. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser. Chester Joshua V. Salmi DVM The study was conducted to determine the antibiotic sensitivity profile of Salmonella spp, isolates in two hundred chicken gallbladder samples taken fern a dressing plant in Trece Martirez City, Cavite. One hundred and sixty five colonies were isolated from Lysine Desoxycholate agar or XLD and were characterized culturally appearing as red colonies with or without black centers. These isolates were characterized morphologically and 157 were gram negative and rod shaped orgy inns_ Biochemically, the isolates was found to possess the following Salmonella spp., reactions: Oxidate TiPoative, alkaline slant and acid butt with hydrogen sulfide and gas formation on Triple Iron Sum (TS1) agar, produces hvdrogen sulfate and having negative results on Indole and shows motility on Sulfide Indole Motility (SIM) Methyl Red positive and Voues-Proskaeur test neffative_ positive for both Citrate and Nitrate WEm tests and negative for the Urease and Gelatinase tests. The isolate also fermented u tic k.lse and maltose but not lactose. The isolate was considered as PresumPlive Sdlizonella spp., and were further tested serologic ally using Polyvalent 0 (A-1) and Vi and Vi antiserum. Results showed that one isolate agglutinated the Polyvalent 0 (A-1)) amd Vi antiserum and negative Vi agglutination. The isolate was found to be susceptible ciprofloxacin, fosfomycin, amoxicillin, gentamycin, trimeghoprim-sulfamethoxazole, doxvcycline and cephalothin but were resistant to lincomvcin. Intermediate result was observed from nitrofurantoin. The isolation rate of Salmonella spp., in the gallbladder of chickens from a dressing plant in Trece Martirez City, Cavite, was found to be 0.6%. Regardless of its low result proper handling and processing should always be observed and followed to avoid accidental cross-contamination from the giblets to carcass to ensure public safety and that indiscriminate usage of antibiotics be avoided

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	636.513 B32e 2009 (Browse shelf(Opens below))		Link to resource	Room use only	T-3945		00007821

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

BATILES, ELLEN GRACE M., Cavite State University, Indang, Cavite, April 2009. Antibiotic Sensitivitv Profile of Salmonella spp. isolates in the gallbladder of chicken (Gallus doinesticus, Lim) from a Dressing Plant in Trece Martirez City, Cavite. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser. Chester Joshua V. Salmi DVM

The study was conducted to determine the antibiotic sensitivity profile of Salmonella spp, isolates in two hundred chicken gallbladder samples taken fern a dressing plant in Trece Martirez City, Cavite. One hundred and sixty five colonies were isolated from Lysine Desoxycholate agar or XLD and were characterized culturally appearing as red colonies with or without black centers.

These isolates were characterized morphologically and 157 were gram negative and rod shaped orgy inns_ Biochemically, the isolates was found to possess the following Salmonella spp., reactions: Oxidate TiPoative, alkaline slant and acid butt with hydrogen sulfide and gas formation on Triple Iron Sum (TS1) agar, produces hvdrogen sulfate and having negative results on Indole and shows motility on Sulfide Indole Motility (SIM) Methyl Red positive and Voues-Proskaeur test neffative_ positive for both Citrate and Nitrate WEm tests and negative for the Urease and Gelatinase tests. The isolate also fermented u tic k.lse and maltose but not lactose. The isolate was considered as PresumPlive Sdlizonella spp., and were further tested serologic ally using Polyvalent 0 (A-1) and Vi and Vi antiserum.

Results showed that one isolate agglutinated the Polyvalent 0 (A-1)) amd Vi antiserum and negative Vi agglutination. The isolate was found to be susceptible ciprofloxacin, fosfomycin, amoxicillin, gentamycin, trimeghoprim-sulfamethoxazole, doxvcycline and cephalothin but were resistant to lincomvcin. Intermediate result was observed from nitrofurantoin. The isolation rate of Salmonella spp., in the gallbladder of chickens from a dressing plant in Trece Martirez City, Cavite, was found to be 0.6%.

Regardless of its low result proper handling and processing should always be observed and followed to avoid accidental cross-contamination from the giblets to carcass to ensure public safety and that indiscriminate usage of antibiotics be avoided

Submitted to the University Library 06/15/2009 T-3945