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Cytogenetic behavior of four varieties of banana "bungulan, lakatan, latundan, and saba" / by Riznel C. Cresino, Mailany M. Papa and Korina B. Ynion.

By:

Cresino, Riznel C [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2005.Description: xiv, 68 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

634.772 C86 2005

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

Science High School, College of Education (CED)

Abstract: CRESINO, RIZNEL C.; PAPA, MAILANY M.; YNION, KORINA B. Cytogenetic Behavior of Four Varieties of Banana (Musa sp. Colla): ‘Bungulan’, ‘Lakatan’, ‘Latundan’, and ‘Saba’. Cavite State University, Science High School, Indang, Cavite. April 2005. Adviser: Ms. Miriam C. Du. Co- adviser: Prof. Dulce L. Ramos. This study was generally conducted to characterize cytogenetically the four varieties of banana: ‘Bungulan’, ‘Lakatan’, ‘Latundan’, and ‘Saba’. Specifically, it aimed to: describe the chromosomal configurations observed during mitosis and meiosis; determine the mitotic indices for the four varieties of banana; describe the abnormal configurations; and evaluate the pollen fertility of the four varieties of banana. Anthers of ‘Bungulan’, ‘Lakatan’, ‘Latundan’, and ‘Saba’ were used to study meiosis. On the other hand, their root tips were used to study mitosis. The anthers and root tips were fixed in freshly prepared Farmer’s solution (1 part glacial acetic acid: 3 parts 95% Ethyl alcohol) between 11:00 - 12:00 noon. The usual acetocarmine squash technique was applied to samples to examine cell division. Results showed that the Mitotic Index (MI) ranged from 75.28 to 81.91 percent. This high percentage implies that there is a high number of cells available for cell division and consequently; more cells are available for growth and development, maintenance and repair of the organism. In meiosis, ‘Latundan’ obtained a total of 32.12 percent of cells undergoing meiosis I followed by ‘Saba’ with 31.66 percent of cells and ‘Lakatan’ with 30.29 percent of cells. ‘Bungulan’ had the least percentage of cells (29.20%) undergoing meiosis I. ‘Bungulan’ had the highest total percentage of cells (32.14%) undergoing meiosis II while ‘Latundan’ had the lowest with 26. 59 percent of cells. Pollen fertility was determined to be relatively high except in ‘Lakatan’. The results of pollen fertility signify that the higher the abnormal chromosome behavior, the lower its percent fertility and vice-versa.

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Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	634.772 C86 2005 (Browse shelf(Opens below))		Link to resource	Room use only	R-472		00000942

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Research Study (General Science Curriculum) Cavite State University.

Includes bibliographical references.

Science High School, College of Education (CED)

CRESINO, RIZNEL C.; PAPA, MAILANY M.; YNION, KORINA B. Cytogenetic Behavior of Four Varieties of Banana (Musa sp. Colla): ‘Bungulan’, ‘Lakatan’, ‘Latundan’, and ‘Saba’. Cavite State University, Science High School, Indang, Cavite. April 2005. Adviser: Ms. Miriam C. Du. Co- adviser: Prof. Dulce L. Ramos.

This study was generally conducted to characterize cytogenetically the four varieties of banana: ‘Bungulan’, ‘Lakatan’, ‘Latundan’, and ‘Saba’. Specifically, it aimed to: describe the chromosomal configurations observed during mitosis and meiosis; determine the mitotic indices for the four varieties of banana; describe the abnormal configurations; and evaluate the pollen fertility of the four varieties of banana.

Anthers of ‘Bungulan’, ‘Lakatan’, ‘Latundan’, and ‘Saba’ were used to study meiosis. On the other hand, their root tips were used to study mitosis. The anthers and root tips were fixed in freshly prepared Farmer’s solution (1 part glacial acetic acid: 3 parts 95% Ethyl alcohol) between 11:00 - 12:00 noon. The usual acetocarmine squash technique was applied to samples to examine cell division.

Results showed that the Mitotic Index (MI) ranged from 75.28 to 81.91 percent. This high percentage implies that there is a high number of cells available for cell division and consequently; more cells are available for growth and development, maintenance and repair of the organism.

In meiosis, ‘Latundan’ obtained a total of 32.12 percent of cells undergoing meiosis I followed by ‘Saba’ with 31.66 percent of cells and ‘Lakatan’ with 30.29 percent of cells. ‘Bungulan’ had the least percentage of cells (29.20%) undergoing meiosis I.

‘Bungulan’ had the highest total percentage of cells (32.14%) undergoing meiosis II while ‘Latundan’ had the lowest with 26. 59 percent of cells.

Pollen fertility was determined to be relatively high except in ‘Lakatan’. The results of pollen fertility signify that the higher the abnormal chromosome behavior, the lower its percent fertility and vice-versa.

Submitted to the University Library R-472