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Molecular detection of Anaplasma marginale in cattle from selected farm in Cavite using ploymerase chain reaction / Lennox Pinili Tapawan.

By:

Tapawan, Lennox Pinili [author]

Contributor(s):

Mago, Emmanuel R [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiii , 50 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

636.2 T16 2018

Online resources:

View Abstract

Production credits:

College of Veterinary Medicine and Biomedical Sciences

Abstract: TAPAWAN, LENNOX P. Molecular Detection of Anaplasma marginale in Cattle from Selected Farms in Cavite Using Polymerase Chain Reaction. Undergraduate Thesis, Doctor of Veterinary Medicine, Cavite State Unversity, Indang, Cavite. May 2018. Adviser: Emmanuel R. Mago DVM, MS The study was conducted to detect the presence of Anaplasma marginate in cattle from selected farms in Cavite. One hundred blood samples of cattle were collected from different municipalities in Cavite. The template DNA was extracted using the PREP DNA/RNA kit (Life Science, Germany). Mammalian actin gene served as internal control of the study. DNA samples positive for Anaplasma app. was further screened for A. marginate using A. marginate specific primers. The detection rate was correlated to different risk factors such as sex, age, backyard or commercial, type of feeding operation, breed, topography, and acaricidal treatment. It was found out that there is a 66 % detection rate of Anaplasma marginate in Cavite. Based on the results of the study, there is a significant association between three topographical areas in Cavite with the lowland areas having the highest detection rate (88%). However, no association between different age groups, sex, type of production, type of feeding management and used of acaricide were observed. The results of the study show that there is high incidence of Anaplasma marginate infections in Cavite. The study also suggests that A. marginate infections in cattle is endemic in Cavite.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	636.2 T16 2018 (Browse shelf(Opens below))		Room use only	T-7852		00077017

Thesis (Doctor of Veterinary Medicine) Cavite State University and Technology.

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences

TAPAWAN, LENNOX P. Molecular Detection of Anaplasma marginale in Cattle from Selected Farms in Cavite Using Polymerase Chain Reaction. Undergraduate Thesis, Doctor of Veterinary Medicine, Cavite State Unversity, Indang, Cavite. May 2018. Adviser: Emmanuel R. Mago DVM, MS
The study was conducted to detect the presence of Anaplasma marginate in cattle from selected farms in Cavite. One hundred blood samples of cattle were collected from different municipalities in Cavite. The template DNA was extracted using the PREP DNA/RNA kit (Life Science, Germany). Mammalian actin gene served as internal control of the study. DNA samples positive for Anaplasma app. was further screened for A. marginate using A. marginate specific primers. The detection rate was correlated to different risk factors such as sex, age, backyard or commercial, type of feeding operation, breed, topography, and acaricidal treatment. It was found out that there is a 66 % detection rate of Anaplasma marginate in Cavite. Based on the results of the study, there is a significant association between three topographical areas in Cavite with the lowland areas having the highest detection rate (88%). However, no association between different age groups, sex, type of production, type of feeding management and used of acaricide were observed. The results of the study show that there is high incidence of Anaplasma marginate infections in Cavite. The study also suggests that A. marginate infections in cattle is endemic in Cavite.

Submitted to the University Library March 03, 2019 T-7852