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Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from selected fermented fruits & vegetables through 16s rRNA gene sequencing / by Aarhus Wayne Nadine Calderon, Princess Catherine G. Catigan, and Kristian Dominic M. Zafico.

By:

Calderon, Aarhus Wayne Nadine [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2022Description: xv, 91 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

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volume

Subject(s):

DDC classification:

579.30 C12 2022

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College of Arts and Sciences (CAS) - Department of Biological Sciences.

Abstract: ZAFICO, KRISTIAN DOMINIC M., CATIGAN, PRINCESS CATHERINE G., CALDERON, AARHUS WAYNE NADINE. Molecular Identification and Phylogenetic Analysis of Lactic Acid Bacteria isolated from Fermented Fruits and Vegetables through 16S rRNA Gene Sequencing. Undergraduate Thesis, Bachelor of Science in Biology, Major in Genetics, Cavite State University, Indang, Cavite. December 2022. Adviser: Dr. Yolanda A. llagan This study was conducted to identify lactic acid bacteria isolated from selected fermented fruits and vegetables through 16S rRNA gene sequencing. It was conducted at the Microbiology Laboratory of the Research Center, Cavite State University, Indang Cavite from July 2022 to December 2022. Twenty-nine presumptive LAB were isolated from selected fermented fruits and vegetables. Twenty selected LAB isolates were then subjected to phenotypic characterization and were classified according to their presumptive genera. The twenty selected isolates were submitted for gene sequencing but only twelve were identified using 16S rRNA gene sequence analysis. Phylogenetic analysis was performed to investigate the relationship between the LAB isolates and reference strains from different fermented foods in Asia. Based on their phenotypic characteristics, the LAB isolates were classified as Enterococcus (2 isolates), Pediococcus (2), and Lactobacillus (16). 16S rRNA gene sequence analysis identified 8 isolates on species level, namely: Lactobacillus plantarum (4 isolates), Pediococcus pentosaceus (2), Enterococcus hirae, and Enterococcus faecalis. Four isolates were identified as Lactobacillus spp. due to the low quality of their nucleotide sequences. Phylogenetic analysis showed close relationships of 7 isolated LAB with reference strains isolated from different fermented foods which have been investigated for probiotic potential.

List(s) this item appears in: Theses 2024

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Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	579.30 C12 2022 (Browse shelf(Opens below))		Link to resource	Room use only	T-9484		00084374

Thesis (Bachelor of Science in Biology major in Genetics) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS) - Department of Biological Sciences.

ZAFICO, KRISTIAN DOMINIC M., CATIGAN, PRINCESS CATHERINE G., CALDERON, AARHUS WAYNE NADINE. Molecular Identification and Phylogenetic Analysis of Lactic Acid Bacteria isolated from Fermented Fruits and Vegetables through 16S rRNA Gene Sequencing. Undergraduate Thesis, Bachelor of Science in Biology, Major in Genetics, Cavite State University, Indang, Cavite. December 2022. Adviser: Dr. Yolanda A. llagan
This study was conducted to identify lactic acid bacteria isolated from selected fermented fruits and vegetables through 16S rRNA gene sequencing. It was conducted at the Microbiology Laboratory of the Research Center, Cavite State University, Indang Cavite from July 2022 to December 2022.
Twenty-nine presumptive LAB were isolated from selected fermented fruits and vegetables. Twenty selected LAB isolates were then subjected to phenotypic characterization and were classified according to their presumptive genera. The twenty selected isolates were submitted for gene sequencing but only twelve were identified using 16S rRNA gene sequence analysis. Phylogenetic analysis was performed to investigate the relationship between the LAB isolates and reference strains from different fermented foods in Asia.
Based on their phenotypic characteristics, the LAB isolates were classified as Enterococcus (2 isolates), Pediococcus (2), and Lactobacillus (16). 16S rRNA gene sequence analysis identified 8 isolates on species level, namely: Lactobacillus plantarum (4 isolates), Pediococcus pentosaceus (2), Enterococcus hirae, and Enterococcus faecalis. Four isolates were identified as Lactobacillus spp. due to the low quality of their nucleotide sequences. Phylogenetic analysis showed close relationships of 7 isolated LAB with reference strains isolated from different fermented foods which have been investigated for probiotic potential.

Submitted to the University Library February 21, 2023 T-9484