Isolation and cytotoxity of the chemical constituents from the bark and sap of kaong (Arenga pinnata) / by Edliz Gale T. Alas.
Material type:
TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2015.Description: xvi, 62 pages : 28 cm. illustrations ; Content type: - text
- unmediated
- volume
- 633.68 Al1 2015
- Science High School
| Item type | Current library | Collection | Call number | Materials specified | URL | Status | Notes | Date due | Barcode |
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 633.68 Al1 2015. (Browse shelf(Opens below)) | Link to resource | Room use only | RS-734 | 00009326 |
Research Study (Science High School) Cavite State University
Includes bibliographical references.
Science High School
ALAS, EDLIZ GALE T., MONSANTO, PENIEL JABEZ E., NOVERO, CYBIL JANE L. Isolation and Cytotoxicity of the Chemical Constituents from the Bark and Sap of Kaong (Arenga pinnata). A Research Study (General Science Curriculum), Science High School, College of Education, Cavite State University, Indang, Cavite, April 2015. Adviser: Prof. Agnes B. Alimboyoguen.
This study entitled "Isolation and Cytotoxicity of the chemical constituents from the Bark and Sap of Kaong (Arenga pinnata)" was conducted to (1) determine the physical properties of the DCM fractions and isolates of the Kaong bark in terms of color, odor, texture, solubility (using the solvents dichloromethane, petroleum ether, and methanol); (2) determine the same physical properties of the Kaong sap; (3) determine the phytochemical components present in each DCM fraction and isolate of the Kaong bark (glycosides, terpenoids, saponins and flavonoids); (4) components present in the Kaong sap; and (5) determine the DCM fraction and isolate which have the highest cytotoxicity effect.
In terms of the Kaong bark fraction's physical properties, it was determined in the
study that the fractions have color varying in intensity in the range of white to green. The fractions were odourless with a powdery texture and determined soluble in dichloromethane. The cytotoxicity test showed that the fraction from 70 to 80 percent Acetone in DCM exhibited the highest percent mortality with 77.78 percent.
As for the Kaong sap's physical properties, the sample had a white-yellowish color, a strong, unpleasant odor, and a texture slightly thicker than water. It was soluble in methanol. Kaong sap had an average of 22.22 percent mortality.
The phytochemical tests revealed that Kaong sap and all the fractions of Kaong
bark were positive of glycosides and terpenoids Furthermore, two isolates, A and B, were obtained from the bark fractions of Kaong which were both soluble in DCM and Petroleum Ether. Phytochemical testing showed that Isolate A was both positive for glycosides and terpenoids while Isolate B was only positive for terpenoids. The cytotoxicity of the isolates was tested with three trials for each isolate using the brine shrimp test. The average percent mortality of the three trials of Isolate A was 88.89 percent while the average percent mortality of the three trials from Isolate B was95.56 percent. This study recommends that the samples (fraction and isolates) be further purified in order to isolate the component. This study also recommends that the isolates be subjected to HPLC in order to quantify the components present.
Submitted copy to the University Library. 08/18/2015 RS-734