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Isolation and characterization of bacteria producing lactase / by Louie Angela B. Caimbon.

By:

Caimbon, Louie Angela B [author]

Contributor(s):

Matel, Hosea L [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xiv, 61 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

579.3 C12 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: CAIMBON, LOUIE ANGELA B. Isolation and Characterization of Bacteria Producing Lactase. Undergraduate thesis. Bachelor of Science in Biology, major in Microbiology. College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2017. Adviser: Dr. Hosea L. Matel This study aimed to isolate and identify lactase-producing bacteria from different raw milk and characterize the produced lactase. In this study, bacteria were isolated from cow, carabao, and goat raw milk obtained from Dasmarifias and Indang, Cavite. Fifteen isolates were obtained from raw milk using MacConkey agar. A total of 4 isolates were lactase-producer based on lactase assay. Most of the lactase activity on both supernatant and cell reached the highest point at 48 hours and down at 72 hours. Isolate 5COB2 (obtained from cow) produced highest lactase activity among the isolates at 48 hours at room temperature, also much higher compared to those observed at 370Celsius. Isolate 5COB2 might produce extracellular lactase due to the observed high lactase activity in the supernatant..Morphological and physiological characteristics of the isolates were studied to identify the organisms. All the four isolates were identified as Klebsiella oxytoca based on the following characteristics; gram negative (-) reaction, rod shaped, positive in VP test, citrate utilization and indole, and negative in hydrogen sulfide production

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	579.3 C12 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6876		00011234

Thesis (BS Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

CAIMBON, LOUIE ANGELA B. Isolation and Characterization of Bacteria Producing Lactase. Undergraduate thesis. Bachelor of Science in Biology, major in Microbiology. College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2017. Adviser: Dr. Hosea L. Matel
This study aimed to isolate and identify lactase-producing bacteria from different
raw milk and characterize the produced lactase. In this study, bacteria were isolated from cow, carabao, and goat raw milk obtained from Dasmarifias and Indang, Cavite.
Fifteen isolates were obtained from raw milk using MacConkey agar. A total of 4 isolates were lactase-producer based on lactase assay. Most of the lactase activity on both
supernatant and cell reached the highest point at 48 hours and down at 72 hours. Isolate 5COB2 (obtained from cow) produced highest lactase activity among the isolates at 48
hours at room temperature, also much higher compared to those observed at 370Celsius.
Isolate 5COB2 might produce extracellular lactase due to the observed high lactase activity in the supernatant..Morphological and physiological characteristics of the isolates were studied to identify the organisms. All the four isolates were identified as Klebsiella oxytoca based on the following characteristics; gram negative (-) reaction, rod shaped, positive in VP test, citrate utilization and indole, and negative in hydrogen sulfide production

Submitted copy to the University Library. 08/03/2017 T-6876