Laboratory techniques in biotechnology : methods and applications / Dr. K. P. Singh.

By:

Singh, K. P [author]

Material type: Text

TextLanguage: English Publication details: New Delhi, India : Random Publications, 2017Description: 302 pages : illustrations ; 24 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

ISBN:

9789386355126 (hardcover)

Subject(s):

Biotechnology -- Study and teaching -- India

LOC classification:

TP248.22 Si6 2017

Online resources:

Click here to view the table of contents

Contents:

Introduction -- Laboratory to field -- Laboratory techniques in biotechnology -- Recombinant DNA technology -- Patenting hybrids and chimeras -- Stem cell technology -- DNA and RNA -- Environment and biological treatment technology -- Biotechnology and genomics sequence.

Summary: "Genetic engineering deals with synthesis of artificial gene, repair of gene, combining of DNA from two organism (recombinant DNA) and manipulating the artificial gene together with the recombinant DNA for the improvement of microbes, plants, animals & human being. Stem cell technology is a rapidly developing field that combines the efforts of cell biologists, geneticists, and clinicians and offers hope of effective treatment for a variety of malignant and non-malignant diseases. Stem cells are defined as totipotent progenitor cells capable of self renewal and multilineage differentiation. 1. Stem cells survive well and show stable division in culture, making them ideal targets for in vitro manipulation. To study or manipulate nucleic acids, the DNA or RNA must first be isolated or extracted from the cells. This can be done through various techniques. Most nucleic acid extraction techniques involve steps to break open the cell and use enzymatic reactions to destroy all macromolecules that are not desired (such as degradation of unwanted molecules and separation from the DNA sample). Cells are broken using a lysis buffer (a solution that is mostly a detergent); lysis means “to split”. These enzymes break apart lipid molecules in the membranes of the cell and the nucleus. Macromolecules are inactivated using enzymes such as proteases that break down proteins, and ribonucleases (RNAses) that break down RNA. His book provide adequate background and current information on recent biotechnological tools and techniques and its application. It will be very useful for a variety of scholars, biotechnologists, medical doctors, researchers, teachers and students in the field of biotechnology."--Back cover

List(s) this item appears in: Print Books 2022

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Holdings
Item type	Current library	Collection	Call number	Materials specified	Status	Notes	Date due	Barcode
Books	Ladislao N. Diwa Memorial Library Reserve Section	Non-fiction	RUS TP248.22 Si6 2017 (Browse shelf(Opens below))		Room use only	78119		00079066

Includes bibliographical references and index.

"Genetic engineering deals with synthesis of artificial gene, repair of gene, combining of DNA from two organism (recombinant DNA) and manipulating the artificial gene together with the recombinant DNA for the improvement of microbes, plants, animals & human being. Stem cell technology is a rapidly developing field that combines the efforts of cell biologists, geneticists, and clinicians and offers hope of effective treatment for a variety of malignant and non-malignant diseases. Stem cells are defined as totipotent progenitor cells capable of self renewal and multilineage differentiation. 1. Stem cells survive well and show stable division in culture, making them ideal targets for in vitro manipulation. To study or manipulate nucleic acids, the DNA or RNA must first be isolated or extracted from the cells. This can be done through various techniques. Most nucleic acid extraction techniques involve steps to break open the cell and use enzymatic reactions to destroy all macromolecules that are not desired (such as degradation of unwanted molecules and separation from the DNA sample). Cells are broken using a lysis buffer (a solution that is mostly a detergent); lysis means “to split”. These enzymes break apart lipid molecules in the membranes of the cell and the nucleus. Macromolecules are inactivated using enzymes such as proteases that break down proteins, and ribonucleases (RNAses) that break down RNA. His book provide adequate background and current information on recent biotechnological tools and techniques and its application. It will be very useful for a variety of scholars, biotechnologists, medical doctors, researchers, teachers and students in the field of biotechnology."--Back cover

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