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Molecular detection of Babesia spp. in cattle from selected farms in Cavite using polymerase chain reaction / by Lorgen C. Medrano.

By:

Medrano, Lorgen C [author]

Contributor(s):

Mago, Emmanuel D [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2018.Description: xiii, 63 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

595.4 M46 2018

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health

Abstract: MEDRANO, LORGEN C. Molecular Detection of Babesia Spp. In Cattle From Selected Farms In Cavite Using Polymerase Chain Reaction, Undergraduate Thesis. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite, December 2018, Adviser: Emmanuel R. Mago, DVM, MS. Babesiosis is caused by tick-borne, protozoan hemoparasites of the genus Babesia. Babesia bigernina and Babesia bovis are the main agents responsible for bovine babesiosis. Those agent do not survive outside its hosts and can only be transmitted through a tick vector. The main vectors of Babesia are Rhipicephalus spp. A total of one hundred (100) whole blood samples were collected from cattle in the different farms from the province of Cavite. DNA samples from whole blood were extracted using the PREP DNA/RNA kit (Life Science, Germany). All one hundred (100) whole blood samples were tested positive for mammalian actin gene that were served as the internal control of the study. Out of the one hundred (100) whole blood samples positive for mammalian actin gene, only three (3) samples tested positive for (Babesia sp.) bearing an amplicon around 1500 bp. The whole blood samples came from a single farm in Brgy. Calubcob Naic, Cavite. The Babesia sp. positive samples were further tested into Babesia bigemina and Babesia bovis specific primers and the results were negative.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	595.4 M46 2018 (Browse shelf(Opens below))		Link to resource	Room use only	T-8040		00077344

Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health

MEDRANO, LORGEN C. Molecular Detection of Babesia Spp. In Cattle From Selected Farms In Cavite Using Polymerase Chain Reaction, Undergraduate Thesis. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite, December 2018, Adviser: Emmanuel R. Mago, DVM, MS.
Babesiosis is caused by tick-borne, protozoan hemoparasites of the genus Babesia. Babesia bigernina and Babesia bovis are the main agents responsible for bovine babesiosis. Those agent do not survive outside its hosts and can only be transmitted through a tick vector. The main vectors of Babesia are Rhipicephalus spp. A total of one hundred (100) whole blood samples were collected from cattle in the different farms from the province of Cavite. DNA samples from whole blood were extracted using the PREP DNA/RNA kit (Life Science, Germany). All one hundred (100) whole blood samples were tested positive for mammalian actin gene that were served as the internal control of the study. Out of the one hundred (100) whole blood samples positive for mammalian actin gene, only three (3) samples tested positive for (Babesia sp.) bearing an amplicon around 1500 bp. The whole blood samples came from a single farm in Brgy. Calubcob Naic, Cavite. The Babesia sp. positive samples were further tested into Babesia bigemina and Babesia bovis specific primers and the results were negative.

Submitted to the University Library June 27, 2019 T-8040