Hemagglutinating activity of birch flower (Broussonetia luzonical) lectin in abo blood group / by Emmanuel A. Berchie, Keithlyn P. Gutierrez, and Abegail P. Rabanzo.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2018.Description: xvi, 87 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 399.0113  B46 2018
Online resources: Production credits:
  • College of Nursing (CON), Department of Medical Technology
Abstract: BERCHIE, EMMANUEL A., GUTIERREZ, KEITHLYN P., RABANZO, ABEGAIL P. Hemagglutinating Activity of Birch flower (Broussonetia luzonica) Lectin in ABO Blood Group. Research Study. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. May 2018, Adviser: Hosea L. Matel, PhD. and Annie M. Ramos, RMT, RN, MD. The extensive application and variety of uses of lectins express the necessity to isolate lectins from indigenous sources since lectins are very expensive. One of the possible native sources of lectin is the birch flower (Broussonetia luzonica) which is commonly known as "himbabao". The study was conducted to determine the presence or absence of hemagglutination in birch flower extracts using microtiter plate (MTP) — based hemagglutination; determine the strength of agglutination of ABO blood antigen through microtiter plate (MTP) — based hemagglutination; characterize the hemagglutinating activity of lectin obtained from birch flower in terms of human blood type specificity and effect of storage time on agglutination activity; and determine significant difference between the microtiter plate (MTP) — based hemagglutination of extracts of O. 15M NaC1 in IX PBS and phosphate buffer saline (PBS) solution. In this research, the lectin was extracted using 0.15M sodium chloride (NaCI) in lX phosphate buffered saline (PBS) solution and PBS solution alone. The isolation and purification of lectin was done by ammonium sulfate precipitation at 40% to 80% saturation. Microliter plate (MTP) P) based hemagglutination using human ABO erythrocytes was performed in order to determine the presence of lectin. Consequently, the birch flower lectin was categorized as a non-blood type specific lectin since it agglutinated all the human blood types A, B, 0 and AB. The 80(') ammonium sulfate precipitate accompanied by 0.15M NaCl in IX 1)13S gave highest titer value among the other extracts with 2048 in type 13 and AB, followed by type A with 1024. The stability of lectin activity was determined after using, protein fractions with one-week, one-month and two-month shelf life. The peak of the lectin activity shown in the study was observed after one month storage of the protein fractions and gradual loss of activity was noted after second month of storage under refrigeration.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 399.0113 B46 2018 (Browse shelf(Opens below)) Link to resource Room use only RS-849 00077064

Thesis (Bachelor of Science in Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON), Department of Medical Technology

BERCHIE, EMMANUEL A., GUTIERREZ, KEITHLYN P., RABANZO, ABEGAIL P. Hemagglutinating Activity of Birch flower (Broussonetia luzonica) Lectin in ABO Blood Group. Research Study. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. May 2018, Adviser: Hosea L. Matel, PhD. and Annie M. Ramos, RMT, RN, MD.

The extensive application and variety of uses of lectins express the necessity to isolate lectins from indigenous sources since lectins are very expensive. One of the possible native sources of lectin is the birch flower (Broussonetia luzonica) which is commonly known as "himbabao". The study was conducted to determine the presence or absence of hemagglutination in birch flower extracts using microtiter plate (MTP) — based hemagglutination; determine the strength of agglutination of ABO blood antigen through microtiter plate (MTP) — based hemagglutination; characterize the hemagglutinating activity of lectin obtained from birch flower in terms of human blood type specificity and effect of storage time on agglutination activity; and determine significant difference between the microtiter plate (MTP) — based hemagglutination of extracts of O. 15M NaC1 in IX PBS and phosphate buffer saline (PBS) solution.

In this research, the lectin was extracted using 0.15M sodium chloride (NaCI) in lX phosphate buffered saline (PBS) solution and PBS solution alone. The isolation and purification of lectin was done by ammonium sulfate precipitation at 40% to 80% saturation. Microliter plate (MTP) P) based hemagglutination using human ABO erythrocytes was performed in order to determine the presence of lectin. Consequently, the birch flower lectin was categorized as a non-blood type specific lectin since it agglutinated all the human blood types A, B, 0 and AB. The 80(') ammonium sulfate precipitate accompanied by 0.15M NaCl in IX 1)13S gave highest titer value among the other extracts with 2048 in type 13 and AB, followed by type A with 1024. The stability of lectin activity was determined after using, protein fractions with one-week, one-month and two-month shelf life. The peak of the lectin activity shown in the study was observed after one month storage of the protein fractions and gradual loss of activity was noted after second month of storage under refrigeration.

Submitted to the University Library May 27, 2019 RS-849

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